Expression of antigens related to hypoxia, stress, and apoptosis in the myocardium after fatal carbon monoxide exposure
Expression of antigens related to hypoxia, stress, and apoptosis in the myocardium after fatal carbon monoxide exposure
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致命一氧化碳暴露后心肌中与缺氧、应激和细胞凋亡相关的抗原的表达
DOI：
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
K. Nishi
中科院分区：
文献类型：
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作者：
Satomu Morita;S. Furukawa;Lisa Wingenfeld;K. Nishi
虽然已经描述并被普遍接受的是，致命的一氧化碳中毒患者的心肌没有显示任何缺血性类型的变化或其他损害，但我们以前用HE和/或AZAN仔细地观察了详细的病理结果。我们对20例一氧化碳中毒和房屋火灾受害者的心肌进行了免疫组织化学研究。我们特别挑选了以前被认为是冷应激抗原的抗体。我们还使用了与应激、细胞凋亡和血管生成相关的抗体。这些核与CIRBP、RBM3和SIRT1抗体具有良好的反应性。虽然CIRBP和RBM3以前被认为是冷应激抗原，但本研究表明，这些抗原也在低氧条件下转录。在一氧化碳中毒患者的心肌中也检测到与应激、细胞凋亡和血管生成相关的抗原。本研究结果提示，CIRBP、RBM3和SIRT1等抗原可能是心肌缺血或缺氧的有用的时间标志物。这项研究还发现，细胞一旦暴露在应激和缺氧中，就会开发和激活信号通路，以防止有害后果，并且在细胞死亡后和死后时间内，表达的抗原可能会保留下来。关键词：一氧化碳中毒、缺氧心肌细胞、CIRBP、RBM3、SIRT1。
Although it has been described and generally accepted that the myocardium does not show any ischemic types of changes or other lesions in fatal carbon monoxide poisoning victims, we previously observed detailed pathological findings with careful staining using HE and/or Azan. We have used immnohistochemistry on the myocardium from twenty victims of both carbon monoxide poisoning and house fire. We especially selected antibodies which were previously recognized as cold stress antigens. And we also used antibodies related to stress, apoptosis, and vascular genesis. The nuclei showed good reactivity with CIRBP, RBM3, and SIRT1 antibodies. Although CIRBP and RBM3 were previously recognized as cold stress antigens, this study indicated that these antigens were also transcribed in hypoxia. Antigens related to stress, apoptosis, and vascular genesis were also detected in the myocardium from carbon monoxide poisoning victims. The results obtained in the present study suggest that antigens, such as CIRBP, RBM3 and SIRT1, may be useful temporal markers of global hypoxia or ischemia in myocardium. This study also found that the cells once exposed to stress and hypoxia, develop and activate signal pathways to prevent harmful consequences, and the expressed antigens may be retained after cell death and during the postmortem interval. Key W ords: Carbon monoxide poisoning, Hypoxic cardiac cells, CIRBP, RBM3, SIRT1.
影响因子：
3.6
作者：
Collard, CD;Lekowski, R;Stahl, GL
通讯作者：
Stahl, GL