Induction of SV40 early transcription by type I interferon.
Induction of SV40 early transcription by type I interferon.
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I 型干扰素诱导 SV40 早期转录。
DOI：
10.1006/excr.1993.1083
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发表时间：
1993
影响因子：
3.7
通讯作者：
Scott,RE
中科院分区：
文献类型：
--
作者：
Tzen,CY;Scott,RE
干扰素（IFN）对许多转化细胞具有癌症抑制活性;然而，IFN并不抑制SV 40大T抗原的转化。因此，本文中描述的研究评估了IFN对3 T3 T细胞中SV 40促进的基因表达的影响。结果表明，无论使用β-半乳糖苷酶还是氯霉素乙酰转移酶（CAT）作为报告基因，I型IFN处理均可诱导SV 40促进的基因表达200%或3倍。这种IFN效应是剂量依赖性的，需要24-48小时的暴露以最大程度地诱导CAT活性，并且可能不是由于IFN对CAT的转录后效应，因为IFN对由胸苷激酶启动子驱动的CAT表达没有影响。然而，IFN诱导SV 40早期转录需要质粒整合到细胞基因组中。另外的数据明确表明，SV 40启动子是IFN作用所必需的，因为如果将SV 40增强子插入胸苷激酶启动子的上游以控制CAT基因的表达，则IFN将不会诱导CAT。
Interferons (IFN) have cancer suppressor activities for many transformed cells; however, IFN does not suppress transformation by SV40 large T antigen. The studies described in this paper therefore evaluated the effect of IFN on SV40-promoted gene expression in 3T3T cells. The results show that SV40-promoted gene expression can be induced 200% or three-fold by Type I IFN treatment regardless of whether β-galactosidase or chloramphenical acetyltransferase (CAT) is used as the reporter gene. This IFN effect is dosage-dependent, requires 24-48 h of exposure for maximum induction of CAT activity, and is probably not due to a post-transcriptional effect of IFN on CAT because IFN has no effect on CAT expression driven by thymidine kinase promoter. The induction of SV40 early transcription by IFN does, however, require that the integration of plasmid within the cell's genome. Additional data specifically show that the SV40 promoter is required for IFN's effect because IFN will not induce CAT if the SV40 enhancer is inserted upstream of thymidine kinase promoter to control expression of CAT gene.