CLATHRIN REQUIREMENT FOR NORMAL GROWTH OF YEAST
CLATHRIN REQUIREMENT FOR NORMAL GROWTH OF YEAST
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DOI：
10.1126/science.3116672
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发表时间：
1987-10-23
期刊：
影响因子：
56.9
通讯作者：
JONES, EW
中科院分区：
文献类型：
--
作者：
LEMMON, SK;JONES, EW
网格蛋白包被的膜和包被的囊泡参与真核细胞不同亚细胞区室之间蛋白质的选择性转移。为了评估网格蛋白在囊泡转运中的作用，启动了酿酒酵母中网格蛋白重链基因（CHCl）的遗传分析。克隆了完整的重链基因，并研究了该基因缺失的影响。无效突变（chcl-Δ）是致命的，除非存在网格蛋白缺乏（scd 1）的抑制剂。即使在抑制基因的存在下，缺乏这种网格蛋白重链的突变体生长缓慢，遗传不稳定，形态异常，并显示出几种酵母功能的丧失或减少。这些结果表明，网格蛋白是酵母正常生长所必需的，因此，很可能是所有真核细胞生长所必需的。
Clathrin-coated membranes and coated vesicles take part in the selective transfer of proteins between different subcellular compartments of eukaryotic cells. To allow assessment of the role of clathrin in vesicular transport, genetic analysis of the clathrin heavy chain gene (CHCl) in Saccharomyces cerevisiae was initiated. The complete heavy chain gene was cloned, and the effects of deletion of this gene were studied. The null mutation (chcl-.DELTA.) is lethal unless a suppressor of clathrin deficiency (scd1) is present. Even in the presence of the suppressor gene, mutants lacking this clathrin heavy chain grow slowly, are genetically unstable, are morphologically abnormal, and show loss of or reduction in several yeast functions. These results indicate that clathrin is required for normal growth of yeast, and, therefore, most likely, for growth of all eukaryotic cells.