Three-dimensional localization precision of the double-helix point spread function versus astigmatism and biplane
Three-dimensional localization precision of the double-helix point spread function versus astigmatism and biplane
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DOI：
10.1063/1.3499652
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发表时间：
2010-10-18
影响因子：
4
通讯作者：
Moerner, W. E.
中科院分区：
文献类型：
--
作者：
Badieirostami, Majid;Lew, Matthew D.;Moerner, W. E.
具有双螺旋点扩散函数 (DH-PSF) 的宽视场显微镜可提供超出光学衍射极限的三维 (3D) 位置信息。我们使用 Fisher 信息分析（包括像素化和不同水平的背景），将 DH-PSF 无偏估计器的理论定位精度与通过像散和双平面成像进行 3D 定位的理论定位精度进行了比较。 DH-PSF 在 2 μm 厚的景深下在所有三个维度上实现几乎恒定的定位精度，而像散和双平面则提高了较小轴向范围内的轴向定位精度。对于高信号背景比，DH-PSF 平均可以实现更好的定位精度。 (C) 2010 年美国物理研究所。 [doi:10.1063/1.3499652]
Wide-field microscopy with a double-helix point spread function (DH-PSF) provides three-dimensional (3D) position information beyond the optical diffraction limit. We compare the theoretical localization precision for an unbiased estimator of the DH-PSF to that for 3D localization by astigmatic and biplane imaging using Fisher information analysis including pixelation and varying levels of background. The DH-PSF results in almost constant localization precision in all three dimensions for a 2 mu m thick depth of field while astigmatism and biplane improve the axial localization precision over smaller axial ranges. For high signal-to-background ratio, the DH-PSF on average achieves better localization precision. (C) 2010 American Institute of Physics. [doi:10.1063/1.3499652]