Smooth muscle cell modulation and cytokine overproduction in varicose veins.: An in situ study
Smooth muscle cell modulation and cytokine overproduction in varicose veins.: An in situ study
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DOI：
10.1002/path.819
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发表时间：
2001-03-01
影响因子：
7.3
通讯作者：
Jacob, MP
中科院分区：
文献类型：
--
作者：
Badier-Commander, C;Couvelard, A;Jacob, MP
静脉曲张疾病的确切病因学和病理生理学仍不清楚。目前工作的目的是原位研究曲张静脉中细胞和基质成分的形态和组成，并与对照静脉进行比较，并确定可能有助于曲张重塑的因素。 Nas 使用的组合组织学、免疫组织化学和生化方法。研究曲张静脉 (n=12) 和对照静脉 (n=9) 的纵向切片，以评估平滑肌细胞的组织、结构和表型；微血管内皮细胞的定位；结缔组织蛋白质的分布；和细胞因子的定位。这些细胞因子通过 ELISA 进一步定量。观察到曲张静脉壁的相当大的异质性，具有连续的肥厚和萎缩节段，呈现内层的严重混乱和许多内膜增厚区域。在肥大部分，内侧平滑肌细胞显示出混乱的改变，表明从收缩性到增殖性和合成表型的调节；此外，滋养管的数量增加。相反，在萎缩部分，细胞和基质成分均减少。当结果以每毫克 DNA 表示时，曲张静脉中 TGF beta1（p 小于或等于 0.005）和 bFGF（p 小于或等于 0.001）增加，VEGF 没有显着改变。这些结果表明，平滑肌细胞的表型调节、细胞外基质代谢的改变和血管生成是导致静脉曲张重塑的形态和功能改变的主要机制。曲张静脉细胞增加的 bFGF 和 TGF beta1 表达可能在静脉壁肥厚中发挥关键作用，但导致动脉瘤扩张的确切机制仍有待阐明 版权所有 (C) 2001 John Wiley & Sons, Ltd.
The exact aetiology and physiopathology of varicose disorders remain unclear. The aim of the present work was to study, in situ, the morphology and composition of cellular and matrix components in varicose veins compared with control veins and to identify factors that could contribute to varicose remodelling. A combined histological, immunohistochemical, and biochemical approach Nas used. Longitudinal sections of varicose (n=12) and control veins (n=9) were studied to assess the organization, structure, and phenotype of smooth muscle cells; the localization of microvascular endothelial cells; the distribution of connective tissue proteins; and the localization of cytokines. These cytokines were further quantified by ELISA. Considerable heterogeneity of the varicose vein wall was observed, with a succession of hypertrophic and atrophic segments, presenting severe disorganization of the medial layer and numerous areas of intimal thickening. In hypertrophic portions, medial smooth muscle cells showed marl;ed alterations suggesting modulation from a contractile to a proliferative and synthetic phenotype; furthermore, the number of vasa vasorum was increased, In contrast, in atrophic portions, both cellular and matrix components were decreased. TGF beta1 (p less than or equal to0.005) and bFGF (p less than or equal to 0.001) were increased and VEGF was not significantly modified in varicose veins when the results were expressed per mg of DNA. These results show that phenotypic modulation of smooth muscle cells, altered extracellular matrix metabolism, and angiogenesis are the main mechanisms contributing to the morphological and functional modifications of varicose remodelling. The increased expression of bFGF and TGF beta1 by varicose vein cells may play a pivotal role in the hypertrophy elf the venous wall, but the exact mechanism leading to aneurysmal dilatations remains to be elucidated Copyright (C) 2001 John Wiley & Sons, Ltd.