Contact solid-phase microextraction with uncoated glass and polydimethylsiloxane-coated fibers versus solvent sampling for the determination of hydrocarbons in adhesion secretions of Madagascar hissing cockroaches Gromphadorrhina portentosa (Blattodea) by gas chromatography-mass spectrometry.
Contact solid-phase microextraction with uncoated glass and polydimethylsiloxane-coated fibers versus solvent sampling for the determination of hydrocarbons in adhesion secretions of Madagascar hissing cockroaches Gromphadorrhina portentosa (Blattodea) by gas chromatography-mass spectrometry.
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使用无涂层玻璃和聚二甲基硅氧烷涂层纤维进行接触固相微萃取与溶剂取样，通过气相色谱-质谱法测定马达加斯加嘶嘶蟑螂 Gromphadorrina portentosa (Blattodea) 粘附分泌物中的碳氢化合物
DOI：
10.1016/j.chroma.2015.02.027
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
M. Lämmerhofer
中科院分区：
文献类型：
--
作者：
H. Gerhardt;C. Schmitt;O. Betz;K. Albert;M. Lämmerhofer
通过气相色谱质谱法研究了Gromphadorhina portentosa（马达加斯加嘶嘶蟑螂，Blattodea）粘附分泌物的分子特征，特别是对直链和支链碳氢化合物的综合分析。为此，对跗骨（脚）的分泌物（可能有助于光滑表面上的粘附）和取自胫骨（小腿）的对照样品进行了分析，并比较了它们的分子指纹，其中含有一般角质层碳氢化合物，这些碳氢化合物被认为不参与生物粘附功能。这项研究的主要分析困难在于从活体昆虫以及跗节和胫骨上微量昆虫分泌物中进行代表性、空间控制、精确和可重复的采样。因此，通过重复测量跗骨和胫骨样品，比较了三种不同的体内采样方法的采样再现性和提取效率。虽然使用聚二甲基硅氧烷 (PDMS) 纤维的接触式固相微萃取 (SPME) 显示出更高的峰强度，但自制的无涂层玻璃纤维在接触式 SPME 采样中具有最佳的重复性。这两种接触固相微萃取采样方法的色谱图在统计学上没有显着差异。个体间差异大于不同采样方法的分子模式中潜在存在的微小差异。通过溶剂萃取进行取样非常耗时，灵敏度较低且重现性较差。一般来说，使用廉价玻璃纤维进行接触式固相微萃取取样是 PDMS-SPME 取样的可行替代方案。睑板粘连分泌物的碳氢化合物模式在性质上与胫骨的表皮碳氢化合物模式相似。然而，跗骨分泌物中的碳氢化合物通常不如胫骨分泌物丰富。
Molecular profiles of adhesion secretions ofGromphadorrhina portentosa(Madagascar hissing cockroach, Blattodea) were investigated by gas chromatography mass spectrometry with particular focus on a comprehensive analysis of linear and branched hydrocarbons. For this purpose, secretions from the tarsi (feet), possibly contributing to adhesion on smooth surfaces, and control samples taken from the tibiae (lower legs), which contain general cuticular hydrocarbons that are supposed to be not involved in the biological adhesion function, were analyzed and their molecular fingerprints compared. A major analytical difficulty in such a study constitutes the representative, spatially controlled, precise and reproducible sampling from a living insect as well as the minute quantities of insect secretions on both tarsi and tibiae. Thus, three different in vivo sampling methods were compared in terms of sampling reproducibility and extraction efficiency by replicate measurement of samples from tarsi and tibiae. While contact solid-phase microextraction (SPME) with a polydimethylsiloxane (PDMS) fiber showed higher peak intensities, a self-made uncoated glass fiber had the best repeatability in contact-SPME sampling. Chromatographic profiles of these two contact-SPME sampling methods were statistically not significantly different. Inter-individual variances were larger than potentially existing minor differences in molecular patterns of distinct sampling methods. Sampling by solvent extraction was time consuming, showed lower sensitivities and was less reproducible. In general, sampling by contact-SPME with a cheap glass fiber turned out to be a viable alternative to PDMS-SPME sampling. Hydrocarbon patterns of the tarsal adhesion secretions were qualitatively similar to those of epicuticular hydrocarbon profiles of the tibiae. However, hydrocarbons were in general less abundant in tarsal secretions than secretions from tibiae.
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