H1N1/09 Influenza A Virus Infection of Immortalized First Trimester Human Trophoblast Cell Lines
H1N1/09 Influenza A Virus Infection of Immortalized First Trimester Human Trophoblast Cell Lines
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DOI：
10.1111/j.1600-0897.2012.01172.x
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发表时间：
2012-09-01
影响因子：
3.6
通讯作者：
Hayakawa, Satoshi
中科院分区：
文献类型：
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作者：
Komine-Aizawa, Shihoko;Suzaki, Ai;Hayakawa, Satoshi
问题尽管在最近的猪源甲型H1N1/09流感（H1N1/09）大流行中报告了高孕产妇死亡率，但其对胎儿-胎盘单位的直接影响尚不清楚。在本研究中，我们研究了永生化人滋养层细胞对2009年H1N1流感A（H1N1/09）病毒临床分离株的易感性。研究方法H1N1/09病毒是从一名流感患者身上分离出来的，经过测序后被鉴定为A/成田/2009（H1N1）毒株。用病毒攻击滋养层细胞系Swan 71和HTR 8，并检测H1N1/09病毒RNA和蛋白的表达。结果接种后24小时左右，病毒RNA和蛋白质出现明显变化。然而，未检测到病毒释放。结论人早孕滋养层细胞系对H1N1/09甲型流感病毒易感。然而，病毒释放和细胞病变效应极小。我们的数据表明H1N1/09病毒对胎盘的损害可能是有限的。
Problem Although high maternal mortality was reported in the most recent pandemic of swine-origin influenza A H1N1/09 (H1N1/09), its direct effects on the feto-placental unit are unknown. In the present study, we examined the susceptibility of immortalized human trophoblasts to clinical isolates of pandemic 2009 H1N1 influenza A (H1N1/09) virus. Method of study The H1N1/09 virus was isolated from a patient with influenza, sequenced and identified as the A/Narita/2009 (H1N1) strain. The trophoblast cell lines Swan71 and HTR8 were challenged with the virus and examined for the expression of H1N1/09 viral RNA and proteins. Results Viral RNA and proteins were observed 24 similar to hr after inoculation. However, viral release was not detected. Conclusion First trimester human trophoblast cell lines were susceptible to the H1N1/09 influenza A virus. However, viral release and cytopathic effects were minimal. Our data suggest that placental damage by the H1N1/09 virus may be limited.