ISOLATION AND DEVELOPMENT OF CHLOROSOMES IN THE GREEN BACTERIUM CHLOROFLEXUS-AURANTIACUS
ISOLATION AND DEVELOPMENT OF CHLOROSOMES IN THE GREEN BACTERIUM CHLOROFLEXUS-AURANTIACUS
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DOI：
10.1128/jb.147.3.1021-1031.1981
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发表时间：
1981-01-01
影响因子：
3.2
通讯作者：
FULLER, RC
中科院分区：
文献类型：
--
作者：
SPRAGUE, SG;STAEHELIN, LA;FULLER, RC
采用冻裂电镜技术，对金针桃光合机构发育过程中叶绿体结构的变化进行了进一步研究。在发育过程中，由于光照强度降低或氧张力降低，每个细胞的叶绿体数量增加。同样的条件也导致叶绿体普遍增厚，但不影响它们的长度和宽度。叶绿体的增厚与细菌叶绿素c/细菌叶绿素a比值的增加是平行的。光合机构的半有氧诱导不能在给定的培养物的所有细胞中产生叶绿体的同步组装。即使相邻的单丝细胞在反应速率和程度上也表现出很大的差异。.apprx的平行外观。5nm颗粒（呈点阵构型）在膜附着部位，结晶基板材料（具有.apprx的周期性）。6 nm)靠近膜附着位点，叶绿体包膜层在纵向定向的棒状元素（直径0.96 nm）之前添加。6nm)到叶绿体核。每个叶绿体都能将能量输送到。apprx。100个反应中心。只有在弱光条件下生长的细胞才能用简单的密度梯度法分离出叶绿体。细菌叶绿素A在790 nm吸收与分离的叶绿体有关。十二烷基硫酸锂-聚丙烯酰胺凝胶电泳显示，叶绿体中只有少数低分子量多肽（< 15,000）。
Freeze-fracture EM was used to study further the changes in chlorosome structure during the development of the photosynthetic apparatus in C. aurantiacus J-10-fl. During development, in response to decreased light intensity or lower O2 tension, the number of chlorosomes per cell increased. The same conditions also led to a general thickening of chlorosomes but did not affect their length or width. The thickening of the chlorosomes paralleled increases in the bacteriochlorophyll c/bacteriochlorophyll a ratio. Semiaerobic induction of the photosynthetic apparatus did not produce a synchronous assembly of chlorosomes in all cells of a given culture. Even adjacent cells of a single filament showed great variations in the rate and extent of response. Parallel appearance of .apprx. 5-nm particles (in a lattice configuration) in the membrane attachment site, the crystalline baseplate material (with a periodicity of .apprx. 6 nm) adjacent to the membrane attachment site, and the chlorosome envelope layer preceded addition of longitudinally oriented, rodlike elements (diameter .simeq. 6 nm) to the chlorosome core. Each chlorosome can funnel energy into .apprx. 100 reaction centers. Chlorosomes could be isolated by a simple density gradient procedure only from cells grown at low light intensity. A bacteriochlorophyll a species absorbing at 790 nm was associated with isolated chlorosomes. Lithium dodecyl sulfate-polyacrylamide gel electrophoresis of chlorosomes showed only a few low MW polypeptides (< 15,000).