Effect of connective tissue growth factor (CCN2/CTGF) on proliferation and differentiation of mouse periodontal ligament-derived cells.
Effect of connective tissue growth factor (CCN2/CTGF) on proliferation and differentiation of mouse periodontal ligament-derived cells.
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DOI：
10.1186/1478-811x-3-11
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发表时间：
2005-10-05
期刊：
影响因子：
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通讯作者：
Takigawa M
中科院分区：
文献类型：
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作者：
Asano M;Kubota S;Nakanishi T;Nishida T;Yamaai T;Yosimichi G;Ohyama K;Sugimoto T;Murayama Y;Takigawa M
已知 CCN2/CTGF 参与牙胚发育和牙周组织重塑，以及间充质组织发育和再生。在本研究中，我们在体外研究了CCN2/CTGF在牙周膜细胞（小鼠牙周膜衍生细胞系：MPL）增殖和分化中的作用。在 MPL 细胞培养物中，CCN2/CTGF 基因的 mRNA 表达在稀疏培养物中比在汇合培养物中更强，并且通过 TGF-β 显着增强。向 MPL 培养物中添加重组 CCN2/CTGF (rCCN2) 会以剂量依赖性方式刺激 DNA 合成和细胞生长。此外，rCCN2的添加还增强了碱性磷酸酶（ALPase）、I型胶原和骨膜素的mRNA表达，后者被认为是骨膜和牙周膜的特异性标志物；而它对典型成骨细胞标志物（例如骨桥蛋白和骨钙蛋白）的 mRNA 表达几乎没有影响。最后，rCCN2/CTGF 还刺激 ALPase 活性和胶原蛋白合成。这些结果共同表明CCN2/CTGF在包括牙周膜在内的牙周组织的发育和再生中发挥重要作用。
CCN2/CTGF is known to be involved in tooth germ development and periodontal tissue remodeling, as well as in mesenchymal tissue development and regeneration. In this present study, we investigated the roles of CCN2/CTGF in the proliferation and differentiation of periodontal ligament cells (murine periodontal ligament-derived cell line: MPL) in vitro. In cell cultures of MPL, the mRNA expression of the CCN2/CTGF gene was stronger in sparse cultures than in confluent ones and was significantly enhanced by TGF-β. The addition of recombinant CCN2/CTGF (rCCN2) to MPL cultures stimulated DNA synthesis and cell growth in a dose-dependent manner. Moreover, rCCN2 addition also enhanced the mRNA expression of alkaline phosphatase (ALPase), type I collagen, and periostin, the latter of which is considered to be a specific marker of the periosteum and periodontium; whereas it showed little effect on the mRNA expression of typical osteoblastic markers, e.g., osteopontin and osteocalcin. Finally, rCCN2/CTGF also stimulated ALPase activity and collagen synthesis. These results taken together suggest important roles of CCN2/CTGF in the development and regeneration of periodontal tissue including the periodontal ligament.