Quantification of angiogenesis by a computerized image analysis system in renal cell carcinoma.
Quantification of angiogenesis by a computerized image analysis system in renal cell carcinoma.
复制标题
通过计算机图像分析系统对肾细胞癌中的血管生成进行量化。
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
J. Kim
中科院分区：
文献类型：
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作者：
Ji Shin Lee;J. Jung;J. Kim
客观化
目的：确定通过计算机图像分析系统定量的肿瘤血管生成是否与肾癌的临床结果相关。
研究设计
应用免疫组织化学方法，对62例肾癌组织切片中的微血管进行CD34标记。用计算机图像分析技术计算平均微血管计数(MMC)和平均微血管面积百分比(MPMA)。
结果
MMC为19.3%~315.0，MPMA为0.6%~17.9%。MMC与MPMA呈高度显著正相关(r=0.867，P<0.01)。尽管MMC和MPMA随核分级和TNM分期的增加而降低，但这一差异并不具有统计学意义。MMC和MPMA的存活率在统计学上没有显著差异。
结论
我们的结果表明，计算机图像分析可以准确地评估肿瘤血管生成，但肾细胞癌的肿瘤血管生成并不能提供肾癌的显著预后信息。
OBJECTIVE
To ascertain whether tumor angiogenesis quantitated by a computerized image analysis system correlates with clinical outcome in renal cell carcinoma.
STUDY DESIGN
Microvessels were immunohistochemically labeled with antibodies to CD34 in sections from 62 cases of renal cell carcinoma. Computerized image analysis was used to evaluate the mean microvessel count (MMC) and mean percentage microvessel area (MPMA).
RESULTS
MMC ranged from 19.3 to 315.0, while MPMA was 0.6-17.9%. There was a highly significant correlation between MMC and MPMA (r = .867, P < .01). Although MMC and MPMA decreased with increasing nuclear grade and TNM stage, this difference failed to achieve statistical significance. No statistically significant differences in survival were found for MMC or MPMA.
CONCLUSION
Our results indicate that computerized image analysis can evaluate accurately tumor angiogenesis, but tumor angiogenesis in renal cell carcinoma does not provide significant prognostic information in renal cell carcinoma.