Involvement of abscisic acid in postharvest water-deficit stress associated with the accumulation of anthocyanins in strawberry fruit
Involvement of abscisic acid in postharvest water-deficit stress associated with the accumulation of anthocyanins in strawberry fruit
复制标题
脱落酸参与与草莓果实花青素积累相关的采后缺水胁迫
DOI：
10.1016/j.postharvbio.2015.08.003
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发表时间：
2016-01-01
影响因子：
7
通讯作者：
Luo, Zisheng
中科院分区：
文献类型：
--
作者：
Chen, Jingxin;Mao, Linchun;Luo, Zisheng
水果在收获后容易遭受缺水胁迫并加速衰老。为了了解采后缺水对草莓果实衰老进程的影响，将果梗浸入水或脱落酸溶液中，并在20℃、相对湿度50%的条件下避光保存。结果表明，不通过花梗供水的离体果，缺水胁迫严重，失重大，含水量低。然而，通过花梗吸水的果实成功地避免了缺水胁迫，且失重和含水量略有变化。缺水显着提高了花青素水平，并增加了 PAL C4H 和 DFR 活性。调节花青素生物合成的转录因子（FaMYB1、FabHLH3 和 FaITG1）的表达在缺水胁迫下也上调。同时，胁迫提高了草莓果实中脱落酸的水平以及FaNCED1和FaASR的表达。此外，外源ABA的施用表现出与缺水胁迫相似的特征。这些结果表明采后缺水会加速草莓果实中花青素的积累，脱落酸可能在应对缺水胁迫中发挥作用。 (C) 2015 年由 Elsevier B.V. 出版
Fruits are prone to suffer water-deficit stress and accelerated senescence after harvest. To understand the effect of postharvest water-deficit on the senescence progress of strawberry fruit, fruit peduncles were dipped into water or abscisic acid solution and stored at 20 degrees C with 50% relative humidity in the dark. The results showed that detached fruit without water supply through the peduncles suffered serious waterdeficit stress with great weight loss and low moisture content. However, fruit absorbing water through their peduncles successfully avoided the water-deficit stress with a slight change of weight loss and moisture content. Water-deficit significantly promoted anthocyanin levels with increased PAL C4H and DFR activities. The expressions of transcriptional factors that regulate a nthocyanin biosynthesis (FaMYB1, FabHLH3 and FaITG1) were also up-regulated under water-deficit stress. Meanwhile, stress elevated the abscisic acid level as well as FaNCED1 and FaASR expression in strawberry fruit. Furthermore, exogenous ABA application exhibited the similar features with water-deficit stress. These results suggested that postharvest water-deficit would accelerate anthocyanin accumulation in strawberry fruit and that abscisic acid may play a role in the response to water-deficit stress. (C) 2015 Published by Elsevier B.V.