Development of novel self-assembled poly(3-acrylamidophenylboronic acid)/poly(2-lactobionamidoethyl methacrylate) hybrid nanoparticles for improving nasal adsorption of insulin
Development of novel self-assembled poly(3-acrylamidophenylboronic acid)/poly(2-lactobionamidoethyl methacrylate) hybrid nanoparticles for improving nasal adsorption of insulin
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DOI：
10.1039/c1sm06085f
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发表时间：
2012
期刊：
影响因子：
3.4
通讯作者：
Cui Cheng;Xinge Zhang;Jixin Xiang;Yanxia Wang;C. Zheng;Zhentan Lu;Chaoxing Li
中科院分区：
文献类型：
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作者：
Cui Cheng;Xinge Zhang;Jixin Xiang;Yanxia Wang;C. Zheng;Zhentan Lu;Chaoxing Li
众所周知，苯基硼酸衍生物与糖具有化学相互作用。因此，通过聚(3-丙烯酰胺苯基硼酸)(pAPBA)的硼酸基团与聚(2-乳糖酰胺基乙基甲基丙烯酸酯)(pLAMA)的羟基之间的共价络合形成具有核壳结构的稳定纳米粒子。透射电子显微镜（TEM）图像显示，纳米颗粒尺寸约为200 nm，呈不规则球体。甲基噻唑基四唑 (MTT) 测定表明纳米颗粒对人结直肠癌 (Caco-2) 细胞无细胞毒性。共焦激光扫描显微镜 (CLSM) 图像显示纳米颗粒可以内化到 Caco-2 细胞中。通过鼻内给药装载胰岛素的纳米颗粒导致血浆葡萄糖水平显着降低，并且组织学评估显示纳米颗粒不会在鼻上皮中形成病变。纳米颗粒是有前途的鼻腔递送肽和蛋白质药物的载体。
It is well-known that the phenylboronic acid derivatives have chemical interactions with sugars. Hence, stable nanoparticles with core-shell structure were formed by the covalent complexation between boronic acid groups of poly(3-acrylamidophenylboronic acid) (pAPBA) and hydroxyl groups of poly(2-lactobionamidoethyl methacrylate) (pLAMA). The image taken by transmission electron microscopy (TEM) shows that the nanoparticles had a size of about 200 nm and were irregular spheres. The methyl thiazolyl tetrazolium (MTT) assay suggests that the nanoparticles were non-cytotoxic on the human colorectal carcinoma (Caco-2) cells. The confocal laser scanning microscope (CLSM) images show that the nanoparticles could internalize into Caco-2 cells. The insulin-loaded nanoparticles by intranasal administration led to a significant decrease in the plasma glucose levels and the histological assessment revealed that the nanoparticles would not develop lesions in the nasal epithelium. The nanoparticles are promising carriers for peptide and protein drugs in nasal delivery.