Development and validation of low-intensity pulsed ultrasound systems for highly controlled in vitro cell stimulation
Development and validation of low-intensity pulsed ultrasound systems for highly controlled in vitro cell stimulation
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DOI：
10.1016/j.ultras.2021.106495
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发表时间：
2021-06-26
期刊：
影响因子：
4.2
通讯作者：
Ricotti, L.
中科院分区：
文献类型：
--
作者：
Fontana, F.;Iberite, F.;Ricotti, L.
这项工作的目的是描述两个低强度脉冲超声刺激系统的开发和验证，能够控制输送到生物靶点的剂量。在压力场形状和强度方面，对高频范围（500 kHz至5 MHz）和低频值（38 kHz）进行了传感器表征。这允许在射束轴上定义在刺激期间生物样本应放置的距离，并精确地知道目标处的强度。精心设计的保留系统被开发出来，用于存放生物样品。密封测试证明了它们对外部污染物的不渗透性。系统的组装/拆卸时间结果类似于3分钟。时域声学模拟允许精确地估计生物样品室内的超声波束，从而能够通过调制换能器的输入电压来精确地控制要传输到生物目标的压力。还进行了生物体外试验，证明了系统的无菌性，以及在多次浸入水中超过7天后对生长细胞没有毒性和炎症作用。
This work aims to describe the development and validation of two low-intensity pulsed ultrasound stimulation systems able to control the dose delivered to the biological target. Transducer characterization was performed in terms of pressure field shape and intensity, for a high-frequency range (500 kHz to 5 MHz) and for a low-frequency value (38 kHz). This allowed defining the distance, on the beam axis, at which biological samples should be placed during stimulation and to exactly know the intensity at the target. Carefully designed retaining systems were developed, for hosting biological samples. Sealing tests proved their impermeability to external contaminants. The assembly/de-assembly time of the systems resulted similar to 3 min. Time-domain acoustic simulations allowed to precisely estimate the ultrasound beam within the biological sample chamber, thus enabling the possibility to precisely control the pressure to be transmitted to the biological target, by modulating the transducer's input voltage. Biological in vitro tests were also carried out, demonstrating the sterility of the system and the absence of toxic and inflammatory effects on growing cells after multiple immersions in water, over seven days.