The use of FITC-conjugated monoclonal antibodies for determination of S-phase cells with fluorescence microscopy.
The use of FITC-conjugated monoclonal antibodies for determination of S-phase cells with fluorescence microscopy.
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使用 FITC 偶联的单克隆抗体通过荧光显微镜测定 S 期细胞。
DOI：
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Nurten Gunduz
中科院分区：
文献类型：
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作者：
Nurten Gunduz
用荧光标记的抗溴脱氧尿苷单克隆抗体（FITC-M-anti-BrdUrd）和荧光显微镜技术，对原发性乳腺肿瘤和特发性血小板减少性紫癜（ITP）细胞中DNA合成细胞进行了检测。用~ 3 HdThd标记法和放射自显影法测定了部分组织中的DNA合成细胞。比较了溴脱氧尿苷标记指数（BrdUrd-LI）和氚标记胸苷标记指数（3 HdThd-LI）的结果。乳腺肿瘤的平均BrdUrd-LI为5.4 +/- 1.0%，平均3 HdThd-LI为5.5 +/-1.1%。同样，从健康供体的单核白细胞获得的标记指数对于BrdUrd-LI和3 HdThd-LI的平均值分别为0.5 +/- 0.1%和0.6 +/- 0.1%。两种方法均可观察到ITP患者外周血单个核细胞增殖率的变化。这种血液学疾病的单核白细胞平均BrdUrd-LI为5.4 +/-0.8%。这些结果表明为6.1 +/-0.8%。这些结果表明，这种相对简单的技术为确定给定细胞群中的DNA合成细胞提供了另一种方法。
A method is modified to determine the DNA synthesizing cells in primary human breast tumors and cells with idiopathic thrombocytopenic purpura (ITP) with FITC-conjugated monoclonal antibody against bromodeoxyuridine (FITC-M-anti-BrdUrd) and fluorescence microscopy. The DNA synthesizing cells were also determined from a portion of the same tissues by classical tritiated thymidine labeling (3HdThd) and autoradiography. The results from bromodeoxyuridine labeling index (BrdUrd-LI) and tritiated thymidine labeling index (3HdThd-LI) obtained from the same tissues were compared. The mean BrdUrd-LI for breast tumor was 5.4 +/- 1.0% and the mean 3HdThd-LI was 5.5 +/- 1.1%. Similarly, the labeling indexes obtained from mononuclear leukocytes of healthy donors had means of 0.5 +/- 0.1% and 0.6 +/- 0.1% for BrdUrd-LI and 3HdThd-LI, respectively. The change in the proliferation rate of mononuclear leukocyte population in the samples obtained from patients with ITP could be observed by both methods. The mean BrdUrd-LI of mononuclear leukocytes for this hematological disorder was 5.4 +/- 0.8%. These results suggest that was 6.1 +/- 0.8%. These results suggest that this relatively simple technique offers an alternative method for determining the DNA synthesizing cells in a given cell population.
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影响因子：
11.2
作者：
B. Fisher;N. Gunduz;E. Saffer
通讯作者：
B. Fisher;N. Gunduz;E. Saffer
雌激素及其受体与大鼠肝脏生长和再生的关系。
影响因子：
11.2
作者：
Fisher,B;Gunduz,N;Saffer,EA;Zheng,S
通讯作者：
Zheng,S
原发肿瘤切除、放射、环磷酰胺或他莫昔芬后肿瘤细胞增殖与 17-荧光素雌酮结合之间的相互关系。
影响因子：
11.2
作者：
Fisher,B;Gunduz,N;Saffer,EA
通讯作者：
Saffer,EA
DOI：
10.1073/pnas.80.18.5573
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DOLBEARE, F;GRATZNER, H;GRAY, JW
通讯作者：
GRAY, JW