Infrared Spectroscopic Studies of Major Cellular Components. Part I: The Effect of Hydration on the Spectra of Proteins
Infrared Spectroscopic Studies of Major Cellular Components. Part I: The Effect of Hydration on the Spectra of Proteins
复制标题
主要细胞成分的红外光谱研究。
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
M. Diem
中科院分区：
文献类型：
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作者：
A. Pevsner;M. Diem
The infrared absorption spectra of two sample proteins as dry, lyophilized powders in KBr pellets, as hydrated proteins in KBr pellets, as hydrated or dehydrated films, and in solution phase are reported. We find an enormous increase in the absorption intensities of the amide I and amide II vibrations between the dry and the hydrated phases. This intensity enhancement was observed for hydration by both H2O and D2O. Furthermore, we observe significant changes in the amide I/amide II intensity ratios when comparing solution spectra acquired in transmission and attenuated total reflection (ATR) modes. Observed intensity changes between dry and hydrated protein samples were interpreted in terms of variations in the dielectric constant of the immediate surroundings of the peptide linkages.
