THE GANGLIOSIDES OF ADULT HUMAN AORTA - INTIMA, MEDIA AND PLAQUE
THE GANGLIOSIDES OF ADULT HUMAN AORTA - INTIMA, MEDIA AND PLAQUE
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DOI：
10.1111/j.1432-1033.1987.tb13343.x
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发表时间：
1987-09-01
期刊：
影响因子：
--
通讯作者：
BERGELSON, LD
中科院分区：
文献类型：
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作者：
PROKAZOVA, NV;OREKHOV, AN;BERGELSON, LD
本文研究了心肌梗塞后死亡者主动脉神经节苷脂的组成、结构和定位。通过高效液相色谱法和高效薄层色谱法对单个神经节苷脂进行纯化，并根据其色谱迁移率、碳水化合物组成、神经氨酸酶水解和甲基化分析进行表征。主要的主动脉神经节苷脂被鉴定为GM 3、GM 1、GD 3、GD 1a和GT 1b。内膜和中膜的神经节苷脂成分存在显着差异，动脉粥样硬化斑块的神经节苷脂分布与未受影响的内膜明显不同。后者的特征是高含量的GD3，一种神经节苷脂，被认为与膜通透性、细胞相互作用、增殖和生长以及抑制非特异性免疫反应有关。低密度脂蛋白结合动脉壁和动脉粥样硬化病变引起的免疫学变化的结果可能的影响进行了讨论。
The composition, structure and localization of gangliosides of aorta taken from subjects who had died after myocardial infarction were studied. Individual gangliosides were purified by high-performance liquid chromatography and high-performance thin-layer chromatography and were characterized on the basis of their chromatographic mobility, carbohydrate composition, neuraminidase hydrolysis and methylation analysis. The main aortic gangliosides were identified as GM3, GM1, GD3, GD1a and GT1b. Significant differences in the ganglioside composition of intima and media were detected and the ganglioside profile of atherosclerotic plaques was found to differ markedly from that of unaffected intima. The latter was characterized by high content of GD3, a ganglioside thought to be associatsed with membrane permeability, cell interaction, adhesiveness and growth and to suppress unspecific immune responses. Possible implications of the results in low-density lipoprotein binding to the arterial wall and in immunological changes induced by atherosclerotic lesions are discussed.