Coordinated gene expression between Trichodesmium and its microbiome over day-night cycles in the North Pacific Subtropical Gyre
Coordinated gene expression between Trichodesmium and its microbiome over day-night cycles in the North Pacific Subtropical Gyre
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DOI：
10.1038/s41396-017-0041-5
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发表时间：
2018-04-01
期刊：
影响因子：
11
通讯作者：
Dyhrman, Sonya T.
中科院分区：
文献类型：
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作者：
Frischkorn, Kyle R.;Haley, Sheean T.;Dyhrman, Sonya T.
Trichodesmium是一种广泛存在的，固定N-2的海洋蓝藻，它驱动新固定的氮和碳输入到它发生的少营养生态系统中。毛霉菌菌落普遍存在于构成不同微生物群的异养细菌中，这种全生毛霉菌之间的相互作用可能影响固定碳和固定氮的命运。对北太平洋副热带环流（NPSG）高频拉格朗日采样期间收集的毛霉菌菌落进行了超转录组测序，以确定毛霉菌宿主与微生物组在昼夜循环中可能存在的相互作用。在这里，我们展示了宿主和微生物组之间显著协调的基因表达模式，其中许多具有显著的昼夜周期性。共表达基因的功能表明，全息生物体内与氮、碳和铁等关键资源存在一系列相互作用。在共表达模式中也检测到微生物组依赖于木霉衍生的维生素B12的证据，强调了一种依赖性，可以塑造全息生物群落结构。总的来说，这些表达模式表明，生物相互作用可能影响氮和维生素B12等资源的菌落循环，并将氮-2固定等活动与木霉生理生态的典型非生物驱动因素分离。
Trichodesmium is a widespread, N-2 fixing marine cyanobacterium that drives inputs of newly fixed nitrogen and carbon into the oligotrophic ecosystems where it occurs. Colonies of Trichodesmium ubiquitously occur with heterotrophic bacteria that make up a diverse microbiome, and interactions within this Trichodesmium holobiont could influence the fate of fixed carbon and nitrogen. Metatranscriptome sequencing was performed on Trichodesmium colonies collected during high-frequency Lagrangian sampling in the North Pacific Subtropical Gyre (NPSG) to identify possible interactions between the Trichodesmium host and microbiome over day-night cycles. Here we show significantly coordinated patterns of gene expression between host and microbiome, many of which had significant day-night periodicity. The functions of the coexpressed genes suggested a suite of interactions within the holobiont linked to key resources including nitrogen, carbon, and iron. Evidence of microbiome reliance on Trichodesmium-derived vitamin B12 was also detected in co-expression patterns, highlighting a dependency that could shape holobiont community structure. Collectively, these patterns of expression suggest that biotic interactions could influence colony cycling of resources like nitrogen and vitamin B12, and decouple activities, like N-2 fixation, from typical abiotic drivers of Trichodesmium physiological ecology.