Protein stability: a crystallographer's perspective.
Protein stability: a crystallographer's perspective.
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DOI：
10.1107/s2053230x15024619
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发表时间：
2016-02
期刊：
影响因子：
--
通讯作者：
Rupp B
中科院分区：
文献类型：
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作者：
Deller MC;Kong L;Rupp B
了解蛋白质的稳定性对于优化蛋白质的表达、纯化和结晶至关重要。在这篇综述中，讨论将集中在影响蛋白质稳定性的因素在一定程度上实际的水平，特别是从蛋白质晶体学家的角度。蛋白质稳定性是生物技术、制药和食品工业的主要兴趣话题，也是研究蛋白质的学术研究人员的日常考虑因素。了解蛋白质的稳定性对于优化蛋白质的表达、纯化、配制、储存和结构研究至关重要。在这篇评论中，讨论将集中在影响蛋白质稳定性的因素，在一定程度上实际的水平，特别是从蛋白质晶体学家的角度。蛋白质构象稳定性和蛋白质组成稳定性之间的差异将被讨论，沿着简要介绍了用于分析蛋白质稳定性的关键方法。最后，将讨论在蛋白质表达、纯化和结晶过程中解决蛋白质稳定性问题的策略。
An understanding of protein stability is essential for optimizing the expression, purification and crystallization of proteins. In this review, discussion will focus on factors affecting protein stability on a somewhat practical level, particularly from the view of a protein crystallographer. Protein stability is a topic of major interest for the biotechnology, pharmaceutical and food industries, in addition to being a daily consideration for academic researchers studying proteins. An understanding of protein stability is essential for optimizing the expression, purification, formulation, storage and structural studies of proteins. In this review, discussion will focus on factors affecting protein stability, on a somewhat practical level, particularly from the view of a protein crystallographer. The differences between protein conformational stability and protein compositional stability will be discussed, along with a brief introduction to key methods useful for analyzing protein stability. Finally, tactics for addressing protein-stability issues during protein expression, purification and crystallization will be discussed.