Evaluation of agar and agarose gels for studying mechanical impedance in rice roots
Evaluation of agar and agarose gels for studying mechanical impedance in rice roots
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DOI：
10.1023/a:1004489501678
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发表时间：
1999-01-01
期刊：
影响因子：
4.9
通讯作者：
Barraclough, PB
中科院分区：
文献类型：
--
作者：
Clark, LJ;Whalley, WR;Barraclough, PB
琼脂和琼脂糖凝胶作为机械阻碍水稻（Oryza sativa L.）根的系统进行了评价。使用双层凝胶，使得幼苗在弱凝胶层（0.35%重量/体积）中建立，然后向下生长以遇到高达5.0%（w/v）的处理凝胶。琼脂糖凝胶比相同浓度的琼脂凝胶更强，在5.0%的浓度下达到1.2 MPa的最大抗压强度，而琼脂为0.3 MPa。5.0%的琼脂凝胶刺激伸长的种子轴品种TN 1（与伸长0.2%的凝胶）的幼苗40%，但减少了15%的品种紫胶23。虽然增加琼脂糖浓度降低了两个品种的精轴伸长，但当处理凝胶的浓度大于2.0%时，精轴没有到达处理凝胶的下层。因此，根伸长的减少是一种非机械性抑制。在使用不同批次的琼脂糖进行的实验中，没有观察到这些抑制作用，并且强琼脂糖凝胶刺激了精子轴的伸长。得出的结论是，琼脂和琼脂糖凝胶系统研究不适合研究机械阻抗对水稻根的伸长的影响，并应非常小心地解释使用凝胶作为生长介质的实验结果。
Agar and agarose gels were evaluated as systems to mechanically impede roots of rice (Oryza sativa L.). Two-layer gels were used so that seedlings established in a layer of weak gel (0.35% weight/volume) and then grew downwards to encounter a treatment gel of up to 5.0% (w/v). Agarose gels were stronger than agar gels of the same concentration, reaching a maximum penetrometer resistance of 1.2 MPa at a concentration of 5.0%, compared to 0.3 MPa with agar. The 5.0% agar gel stimulated elongation of the seminal axis by 40% in seedlings of variety TN1 (compared with elongation in the 0.2% gel), but decreased it by 15% in the variety Lac 23. Although increasing agarose concentration decreased seminal axis elongation in both varieties, the seminal axis did not reach the lower layer of treatment gel when the concentration of the treatment gel was greater than 2.0%. The decreased root elongation was therefore a non-mechanical inhibition. In experiments conducted using a different batch of agarose, these inhibitory effects were not seen and strong agarose gels stimulated seminal axis elongation. It was concluded that the agar and agarose gel systems studied were unsuitable for studying the effect of mechanical impedance on the elongation of rice roots and that great care should be taken in interpreting the results of experiments using gels as a growth medium.