Molecular and clinical analyses of the gene encoding the collagen-binding adhesin of Streptococcus mutans
Molecular and clinical analyses of the gene encoding the collagen-binding adhesin of Streptococcus mutans
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DOI：
10.1099/jmm.0.007559-0
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发表时间：
2009-04-01
影响因子：
3
通讯作者：
Ooshima, Takashi
中科院分区：
文献类型：
--
作者：
Nomura, Ryota;Nakano, Kazuhiko;Ooshima, Takashi
变形链球菌是一种已知的龋病致病菌，其主要细胞表面抗原已被广泛研究。最近，一个与I型胶原结合的约120 kDa的CNM蛋白被鉴定出来，并对其编码基因(CNM)进行了克隆和测序。在本研究中，我们对47株不同临床变异链球菌的CNM进行了测序，发现其胶原结合区的核苷酸序列是保守的。我们设计了一种鉴定CNM基因的聚合酶链式反应方法，检测了CNM阳性变形链球菌在不同母子群体中的流行率，并评估了这些菌株对传播和龋齿的意义。21世纪初日本儿童分离的CNM阳性菌株的检出率(8.0%)明显低于80年代分离的菌株(15.8%)(P
Streptococcus mutans is a known pathogen of dental caries and its major cell surface antigens have been widely investigated. Recently, an approximately 120 kDa Cnm protein with binding properties to type I collagen was identified, and its encoding gene (cnm) cloned and sequenced. In the present study, we sequenced cnm from 47 different clinical S. mutans strains and found that the nucleotide alignment of the collagen-binding domain was well conserved. We devised a PCR method for identifying the cnm gene, examined the prevalence of cnm-positive S. mutans strains in various mother-child groups, and assessed the significance of such strains for transmission and dental caries. The detection rate of cnm-positive strains was significantly lower in strains isolated from Japanese children in the 2000s (8.0%) as compared to those isolated in the 1980s (15.8%) (P