STUDIES ON NATURAL ANTIBODIES TO GRAM-NEGATIVE BACTERIA
STUDIES ON NATURAL ANTIBODIES TO GRAM-NEGATIVE BACTERIA
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DOI：
10.1084/jem.115.1.131
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发表时间：
1962-01-01
影响因子：
15.3
通讯作者：
LANDY, M
中科院分区：
文献类型：
--
作者：
MICHAEL, JG;WHITBY, JL;LANDY, M
研究了几种正常血清中革兰氏阴性菌天然抗体的来源、发生和性质。通过基于杀菌反应的程序测量抗体，所述杀菌反应在活性是由测试血清贡献的抗体量的函数的条件下进行。在正常人血清中检测到肠杆菌科7个属的抗体。这些抗体的特异性通过与同源细菌的吸收和通过与纯化的同源体细胞抗原的杀菌活性的抑制来确认。用分级量的细菌悬液进行吸收表明，大量过量的细菌导致抗体的非特异性去除。免疫抗体也有类似的发现。通过定量吸收试验测定，天然抗体和免疫抗体的亲合力无差异。不同物种血清中的天然抗体对热的不稳定性差异很大，但在平行试验中，每个物种的免疫抗体对热的稳定性明显更高。不同物种的幼龄动物中抗体的出现时间和外观各不相同。小鼠在最早的年龄就产生了这些抗体，豚鼠，大鼠和兔子依次出现。无菌大鼠和鸡的血清中没有可证实的大肠杆菌或伤寒沙门氏菌抗体，而在常规条件下饲养的同窝仔的血清中存在这些抗体。另一方面，无菌小鼠和常规小鼠在这些相同抗体的水平方面没有明显差异。
A study was made of the origin, occurrence, and properties of natural antibodies to gram-negative bacteria in the normal serum of several species. Antibody was measured by a procedure based on the bactericidal reaction carried out under conditions in which activity was a function of the amount of antibody contributed by the test serum. Antibody to 7 genera of the family Enterobacteriaceae were demonstrated in normal human serum. The specificity of these antibodies was affirmed by absorption with homologous bacteria and by inhibition of bactericidal activity with purified homologous somatic antigen. Absorption with graded amounts of bacterial suspensions showed that a large excess of bacteria led to nonspecific removal of antibodies. Analogous findings were also made with immune antibody. As determined by quantitative absorption tests no difference could be found in the avidity of natural and immune antibody. Natural antibody in the serum of various species differed considerably as regards their lability to heat, but in parallel tests immune antibody of each species was significantly more heat-stable. The time and appearance of the antibodies varied in young animals, of different species. Mice developed these antibodies at the earliest age, with guinea pigs, rats, and rabbits following in that order. Serum from germ-free rats and chickens aad no demonstrable antibodies to Escherichia coli or Salmonella typhosa whereas these antibodies were present in the serum of litter mates reared under conventional conditions. On the other hand germ-free and conventional mice did not differ appreciably as regards the levels of these same antibodies.