Preparation and evaluation of microfluidic magnetic alginate microparticles for magnetically templated hydrogels.
Preparation and evaluation of microfluidic magnetic alginate microparticles for magnetically templated hydrogels.
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DOI：
10.1016/j.jcis.2019.11.040
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发表时间：
2019-11
影响因子：
9.9
通讯作者：
Ishita Singh;Christopher S Lacko;Zhiyuan Zhao;C. Schmidt;C. Rinaldi
中科院分区：
文献类型：
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作者：
Ishita Singh;Christopher S Lacko;Zhiyuan Zhao;C. Schmidt;C. Rinaldi
我们的目标是开发一种含有多孔微通道的水凝胶支架，该支架模拟复杂的组织微结构，并提供物理线索来引导细胞生长，以实现可扩展的、具有成本效益的组织修复。这些水凝胶通过磁性模板化的新方法进行图案化，其中磁性藻酸盐微粒（MAM）分散在水凝胶前体中，并在水凝胶交联和随后的MAM降解之前在磁场中对齐，留下对齐的多孔结构。在这里，开发了一种使用微流体制造均匀MAMs的方案，以提高模板化微结构的再现性和可调性。通过铁的定量，我们发现，这种方法可以控制磁性氧化铁负载的MAMs。使用布朗动力学模拟和纳米计算机断层扫描的模板水凝胶检查MAM链的长度和对齐，我们发现模拟和测量的MAM链的面密度之间的协议。振荡流变学和应力松弛实验表明，磁模板微通道改变散装水凝胶的机械性能。最后，在体外研究中，大鼠雪旺细胞在模板化水凝胶上培养以模拟外周神经损伤修复，证明了它们沿通道长度沿着提供细胞引导的倾向。我们的研究结果表明，微结构的生物材料，可以帮助组织修复应用的承诺。
Our aim is to develop a hydrogel-based scaffold containing porous microchannels that mimic complex tissue microarchitecture and provide physical cues to guide cell growth for scalable, cost-effective tissue repair. These hydrogels are patterned through the novel process of magnetic templating where magnetic alginate microparticles (MAMs) are dispersed in a hydrogel precursor and aligned in a magnetic field before hydrogel crosslinking and subsequent MAM degradation, leaving behind an aligned, porous architecture. Here, a protocol for fabricating uniform MAMs using microfluidics was developed for improved reproducibility and tunability of templated microarchitecture. Through iron quantification, we find that this approach allows control over magnetic iron oxide loading of the MAMs. Using Brownian dynamics simulations and nano-computed tomography of templated hydrogels to examine MAM chain length and alignment, we find agreement between simulated and measured areal densities of MAM chains. Oscillatory rheology and stress relaxation experiments demonstrate that magnetically templated microchannels alter bulk hydrogel mechanical properties. Finally,in vitrostudies where rat Schwann cells were cultured on templated hydrogels to model peripheral nerve injury repair demonstrate their propensity for providing cell guidance along the length of the channels. Our results show promise for a micro-structured biomaterial that could aid in tissue repair applications.