Quantification and synthesis of cocaine- and amphetamine-regulated transcript peptide (79-102)-like immunoreactivity and mRNA in rat tissues
Quantification and synthesis of cocaine- and amphetamine-regulated transcript peptide (79-102)-like immunoreactivity and mRNA in rat tissues
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DOI：
10.1677/joe.0.1660659
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发表时间：
2000-09-01
影响因子：
4
通讯作者：
Bloom, SR
中科院分区：
文献类型：
--
作者：
Murphy, KG;Abbott, CR;Bloom, SR
The distribution of cocaine- and amphetamine-regulated transcript peptide (79-102)-like immunoreactivity (CART-LI) was quantified in brain and peripheral tissues of male and female Wistar rats, and male obese (fa/fa) and heterozygous (Fa/+) Zucker rats using a specific RIA. CART-LI tissue levels have not been quantified previously. The assay using cocaine- and amphetamine-regulated transcript (CART) (79-102) as a standard and radioactive tracer and an antibody to CART (79-102) fragment, detected CART-LI in all brain regions examined, the anterior and posterior pituitary, the spinal cord and throughout the gastrointestinal tract of both male and female Wistar rats. The highest concentrations were found in the hypothalamus, duodenum, anterior pituitary and posterior pituitary (50.6 +/- 4.4, 26.1 +/- 4.2, 50.0 +/- 1.3 and 373.0 +/- 55.2 pmol/g wet tissue respectively, means +/- S.E.M., n=6-10 male animals). There was no significant variation between the sexes. The concentrations of CART-LI in hypothalami anterior and posterior pituitaries front fa/fa rats were significantly (P
