Computational and functional evaluation of a microfluidic blood flow device
Computational and functional evaluation of a microfluidic blood flow device
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DOI：
10.1097/mat.0b013e3180a5e8ab
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发表时间：
2007-07-01
期刊：
影响因子：
4.2
通讯作者：
Thorsen, Todd
中科院分区：
文献类型：
--
作者：
Gilbert, Richard J.;Park, Hyesung;Thorsen, Todd
支持生理血流的微流体装置的发展有可能产生模拟人体器官功能的生物医学技术。然而，这一领域的进展受到这样一个事实的限制，即为这种装置构建的人工微通道需要实现最大的化学扩散以及血液相容性。为了解决这一问题，我们设计了一个由聚二甲基硅氧烷组成的弹性体微流控装置来模拟肺微循环的几何形状和流动特性。我们的芯片设计的特点是高纵横比（宽度>高度）通道在一个正交互连配置。通过网络设计芯片对血流的有限元模拟表明，视压降随流量呈线性变化。当模拟流速< 250 μ l min（-1）时，模拟压降< 2000 Pa，血流呈层流状，溶血最小。以250 μ l/min灌注6和12小时时的溶血率[血浆总血红蛋白（TPH）（样本对照）/（TPH对照）]计算，在整个装置灌注期间溶血率< 5.0%。在这些灌注条件下，没有证据表明在任何通道段或连接处有显微镜下的血栓。我们得出结论，具有不对称和相互连接的微通道的微流控血流装置具有均匀的流动特性和初步的血液相容性。这种技术应该促进微型氧合器和类似的生物医学设备的发展，这些设备既需要微尺度的反应体积，也需要生理血流量。ASAIO Journal 2007；53:447 - 455。
The development of microfluidic devices supporting physiological blood flow has the potential to yield biomedical technologies emulating human organ function. However, advances in this area have been constrained by the fact that artificial microchannels constructed for such devices need to achieve maximum chemical diffusion as well as hemocompatibility. To address this issue, we designed an elastomeric microfluidic flow device composed of poly (dimethylsiloxane) to emulate the geometry and flow properties of the pulmonary microcirculation. Our chip design is characterized by high aspect ratio (width > height) channels in an orthogonally interconnected configuration. Finite element simulations of blood flow through the network design chip demonstrated that the apparent pressure drop varied in a linear manner with flow rate. For simulated flow rates < 250 mu l min(-1), the simulated pressure drop was < 2000 Pa, the flow was laminar, and hemolysis was minimal. Hemolysis rate, assayed in terms of [total plasma hemoglobin (TPH) (sample - control)/(TPH control)] during 6 and 12 hour perfusions at 250 mu l/min, was < 5.0% through the entire period of device perfusion. There was no evidence of microscopic thrombus at any channel segment or junction under these perfusion conditions. We conclude that a microfluidic blood flow device possessing asymmetric and interconnected microchannels exhibits uniform flow properties and preliminary hemocompatibility. Such technology should foster the development of miniature oxygenators and similar biomedical devices requiring both a microscale reaction volume and physiological blood flow. ASAIO Journal 2007; 53:447-455.