Diurnal rhythm of free estradiol during the menstrual cycle
Diurnal rhythm of free estradiol during the menstrual cycle
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DOI：
10.1530/eje.0.1480227
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发表时间：
2003-02-01
影响因子：
5.8
通讯作者：
Zhou, JN
中科院分区：
文献类型：
--
作者：
Bao, AM;Liu, RY;Zhou, JN
目的：探讨生理周期正常妇女生殖期雌激素的昼夜节律。设计：在月经周期的不同阶段对正常月经周期的健康妇女进行多次唾液取样。方法：对15例月经周期正常的健康妇女，在月经周期的月经期、卵泡晚期/排卵期、黄体前期至中期和黄体晚期，每隔2h采集一次唾液雌二醇，连续24h进行放射免疫测定。采用周期回归法分析唾液雌二醇水平。结果：用非线性周期回归模型对游离雌二醇进行定量分析，发现游离雌二醇具有一定的生物节律。观察到的每日游离雌二醇节律由两个主要部分组成：在整个月经周期中，昼夜节律和昼夜节律在中线（24小时平均水平）、峰宽和振幅方面具有显著的一致性。24小时节律高峰期有在清晨收敛的趋势，而月经期高峰期的发生明显晚于卵泡晚期/排卵期。结论：不同经期雌二醇的昼夜节律具有相似的复杂时间组织。月经周期主要调节昼夜节律的初相。
Objective: To investigate the diurnal rhythm of estrogens in normally cyclic women during reproductive life.Design: Multiple saliva sampling in normally cyclic healthy women during reproductive life at different phases of their menstrual cycles was carried out.Methods: Salivary estradiol was measured by radioimmunoassay in samples collected every 2 h for 24 h from 15 normally cyclic healthy women during reproductive life during the menstrual phase, the late follicular/peri-ovulation phase, the early to mid luteal phase and the late luteal phase, respectively, of their menstrual cycles. The levels of salivary estradiol were analyzed by means of periodic regression.Results: A daily biological rhythm of free estradiol was found after quantification with a nonlinear periodic regression model. The observed diurnal free estradiol rhythm consists of two major components: an asymmetrically peaked diurnal cycle and ultradian harmonics in the range of 6 to 12 h. The diurnal and ultradian rhythms were remarkably consistent throughout the menstrual cycle in terms of mesor (24 h mean level), peak width and amplitude, There was a tendency for the 24-h rhythm acrophases to converge in the early morning, while the acrophase of the menstrual phase occurred significantly later than in the late follicular/peri-ovulation phase.Conclusions: The diurnal rhythm of estradiol has a similar complex temporal organization for different menstrual phases. The menstrual cycle mainly modulates the acrophase of the diurnal rhythm.