Activation of apical chloride channels in the gastric oxyntic cell.
Activation of apical chloride channels in the gastric oxyntic cell.
复制标题
胃泌酸细胞中顶端氯离子通道的激活。
DOI：
10.1126/science.2474200
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Sachs,G
中科院分区：
文献类型：
--
作者：
Demarest,JR;Loo,DD;Sachs,G
从两栖动物Necturus胃粘膜中分离出顶端膜和基底膜之间保留不同形态极性的泌酸细胞。膜片钳技术应用于这些细胞，以确定顶端膜离子通道与盐酸分泌。一类单一的电压依赖性，内向整流氯离子通道中观察到的顶端膜的休息和刺激（酸分泌）泌酸细胞。用二丁酰腺苷3′，5 ′-单磷酸和异丁基甲基黄嘌呤刺激细胞增加通道开放概率，同时增加顶膜表面积。这种氯离子通道可能负责胃粘膜的产电氯离子分泌，也可能参与泌酸细胞的液体和酶分泌功能，类似于在其他外分泌细胞的顶膜中发现的氯离子通道。
Oxyntic cells that retain distinct morphological polarity between apical and basolateral membranes were isolated from the gastric mucosa of the amphibianNecturus. Patch-clamp techniques were applied to these cells to identify apical membrane ion channels associated with hydrochloric acid secretion. A single class of voltage-dependent, inwardly rectifying chloride channels was observed in the apical membranes of both resting and stimulated (acid-secreting) oxyntic cells. Stimulation of the cells with dibutyryladenosine 3′,5′-monophosphate and isobutylmethylxanthine increased channel open probability and simultaneously increased apical membrane surface area. This chloride channel is probably responsible for electrogenic chloride secretion by the gastric mucosa and may also participate in the fluid- and enzyme-secretory functions of the oxyntic cell, analogous to the chloride channels found in the apical membranes of other exocrine cells.