Increased apoptosis in the syncytiotrophoblast in human term placentas complicated by either preeclampsia or intrauterine growth retardation
Increased apoptosis in the syncytiotrophoblast in human term placentas complicated by either preeclampsia or intrauterine growth retardation
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DOI：
10.1067/mob.2002.119176
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发表时间：
2002-01-01
影响因子：
9.8
通讯作者：
Maruo, T
中科院分区：
文献类型：
--
作者：
Ishihara, N;Matsuo, H;Maruo, T
目的：本研究旨在确定先兆子痫和宫内生长迟缓是否与胎盘细胞凋亡增加相关。 研究设计：分析 7 个正常足月胎盘和 7 个并发重度先兆子痫或宫内生长迟缓的足月胎盘的组织标本，用抗生物素蛋白/生物素检测 Fas 抗原和 Bcl-2 蛋白表达。 采用免疫过氧化物酶法检测细胞凋亡，采用末端脱氧核苷酸转移酶脱氧UTP缺口末端标记（TUNEL）法和透射电镜检测细胞凋亡。结果：Fas抗原免疫定位于所有胎盘合体滋养层细胞中，Fas抗原免疫染色强度无变化。 这些胎盘中有明显的合体滋养层。 Bcl-2蛋白在正常足月胎盘的合体滋养细胞中免疫定位丰富，而在伴有严重子痫前期或宫内生长迟缓的足月胎盘中表达最少，细胞滋养细胞和合体滋养细胞细胞核均出现明显的凋亡。重度子痫前期和宫内生长迟缓足月胎盘合体滋养层细胞核凋亡阳性率显着高于正常足月胎盘（重度子痫前期，P < .001；宫内生长迟缓，P < .01）。透射电镜观察重度子痫前期足月胎盘滋养层细胞出现凋亡细胞核。结论：重度子痫前期胎盘合体滋养细胞Bcl-2蛋白表达减少及宫内生长迟缓胎盘合体滋养细胞凋亡增加。
OBJECTIVE: This study was undertaken to determine whether preeclampsia and intrauterine growth retardation are associated with an increase in placental apoptosis.STUDY DESIGN: Tissue specimens from 7 normal term placentas and each of 7 term placentas complicated by severe preeclampsia or intrauterine growth retardation were analyzed, Fas antigen and Bcl-2 protein expression were examined by the avidin/biotin immunoperoxidase method, whereas apoptosis was assessed by the terminal deoxynucleotidyl transferase deoxy-UTP-nick end labeling (TUNEL) method and transmission electron microscopy,RESULTS: Fas antigen was immunolocalized in syncytiotrophoblasts in all placentas examined, No changes in the intensity of Fas antigen immunostaining in syncytiotrophoblasts were apparent among those placentas. Bcl-2 protein was abundantly immunolocalized in syncytiotrophoblasts in normal term placentas, but least abundant in term placentas complicated by severe preeclampsia or intrauterine growth retardation, Apoptosis was apparent in the nuclei of both cytotrophoblasts and syncytiotrophoblasts. The apoptosis positive rate of syncytiotrophoblast nuclei in severe preeclamptic and intrauterine growth retardation term placentas was significantly higher than that in normal term placentas (severe preeclampsia, P < .001; intrauterine growth retardation, P < .01). Transmission electron microscopy revealed the appearance of apoptotic nuclei in trophoblasts in severe preeclamptic term placenta.CONCLUSION: Decreased expression of Bcl-2 protein in syncytiotrophoblasts in severe preeclamptic and intrauterine growth retardation placentas may result in the increase in apoptosis in syncytiotrophoblasts in those placentas.