High affinity D- and L-serine transporter Asc-1: cloning and dendritic localization in the rat cerebral and cerebellar cortices
High affinity D- and L-serine transporter Asc-1: cloning and dendritic localization in the rat cerebral and cerebellar cortices
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DOI：
10.1016/j.neulet.2004.01.014
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发表时间：
2004-03-25
影响因子：
2.5
通讯作者：
Endou, H
中科院分区：
文献类型：
--
作者：
Matsuo, H;Kanai, Y;Endou, H
系统天冬氨酸转运体Asc-1在脑内表达，以高亲和力转运D-丝氨酸和L-丝氨酸。为了确定ASC-1在大鼠脑中的定位，我们分离了ASC-1的大鼠同源基因的cDNA。该编码蛋白命名为RASC-1(大鼠ASC-1)，与小鼠ASC-1(MASC-1)有98%的序列同源性。根据RASC-1和MASC-1的氨基酸序列，制备了两种抗ASC-1的多环抗体，并对大鼠和小鼠的大脑和小脑皮质进行了免疫组织化学分析。ASC-1免疫反应阳性神经元包括小脑浦肯野神经元和新皮质及海马区的锥体神经元。它明显定位于树突和体细胞。Asc-1在脑内的定位表明，Asc-1对脑内氨基酸的动员有重要贡献，包括D-丝氨酸的突触清除和神经元摄取L-丝氨酸，这对浦肯野神经元的生存和树突生长是必不可少的。(C)2004爱思唯尔爱尔兰有限公司。保留所有权利。
System asc transporter Asc-1, expressed in the brain, transports D- and L-serine with high affinity. To determine the localization of Asc-1 in the rat brain, we isolated a cDNA for the rat orthologue of Asc-1. The encoded protein designated as rAsc-1 (rat Asc-1) exhibited 98% sequence identity to mouse Asc-1 (mAsc-1). Based on amino acid sequences of rAsc-1 and mAsc-1, twopolyclonal antibodies against Asc-1 were generated and used for the immunohistochemical analysis on the cerebral and cerebellar cortices of rats and mice. Asc-1 immunoreactivity was detected in neurons, including cerebellar Purkinje neurons and pyramidal neurons in the neocortex and hippocampus. It was clearly localized in dendrites as well as somata. The localization of Asc-1 in brain suggests the significant contribution of Asc-1 to amino acid mobilization in brains including the synaptic clearance of D-serine and the neuronal uptake of L-serine that is essential for survival and dendrite growth of Purkinje neurons in particular. (C) 2004 Elsevier Ireland Ltd. All rights reserved.