Behaviour of Nitinol in osteoblast-like ROS-17 cell cultures
Behaviour of Nitinol in osteoblast-like ROS-17 cell cultures
复制标题
DOI：
10.1016/s0142-9612(01)00143-0
复制
发表时间：
2002-02-01
期刊：
影响因子：
14
通讯作者：
Tuukkanen, J
中科院分区：
文献类型：
--
作者：
Kapanen, A;Ilvesaro, J;Tuukkanen, J
镍钛形状记忆合金镍钛诺（NiTi）已用于牙科器械和胃肠外科手术。镍钛合金是一种很有前途的骨科植入材料，但其生物相容性差。特别是在长期植入中的作用尚未得到证实。我们在细胞培养模型上研究了镍钛合金的作用。进行了与不锈钢、纯钛和纯镍的比较。本文研究了镍钛合金对大鼠骨肉瘤细胞系ROS-17细胞48小时培养过程中细胞死亡率、凋亡率及灶性接触形成的影响。细胞死亡率用钙黄绿素-乙锭-同二聚体联合标记评估。每1000个细胞的死细胞量如下：NiTi中为4个。钛组4.8例，镍组51例。NiTi组和Ti组的死亡细胞数明显低于Ni组（p <0.01）。TUNEL法检测细胞凋亡率。实验结果为：NiTi中1.93个凋亡细胞/1000个细胞。1.1钛组为2.98，镍组为0.62。这两个结果的比较表明，48%的死亡细胞在NIM中是凋亡的，5.6%在Stst中是凋亡的，62%在Ti中是凋亡的，而在Ni组中只有1.8%是凋亡的。用桩蛋白抗体对病灶接触进行染色并计数。与NiTi（774个焦点触点）相比，单位面积的焦点触点数量存在显著差异：Stst为335个（p ≤ 0.01），Ti为462个（p ≤ 0.01），Ni为261个（p ≤ 0.005）。结果表明，NiTi对成骨细胞型ROS-17细胞具有良好的耐受性。(C)2001爱思唯尔科技有限公司版权所有。
Nickel titanium shape memory metal alloy Nitinol (NiTi) has been used in dental wares and in gastrointestinal surgery. Nitinol is a promising implant material in orthopedics, but its biocompatibility. especially in long-term implantation is not confirmed yet. We studied Nitinol's effect Oil a Cell Culture model. Comparisons to stainless steel, pure titanium and pure nickel were performed. The effects of Nitinol on cell death rate, the apoptosis rate and the formation of focal contacts were studied on rat osteosarcoma cell line ROS-17 in 48-h cultures. The cell death rate was assessed with combined calcein-ethidium-homodimer labelling. The amount of dead cells/1000 cells were as follows: four in the NiTi. 21 in the Stst, 4.8 in the Ti and 51 in the Ni group. In the NiTi and Ti groups, the number of dead cells was significantly lower (p less than or equal to0.01) than in Ni group. The rate of apoptosis was detected with TUNEL-assay. The assay results were: 1.93 apoptotic cells/1000 cells in the NiTi. 1.1 in the Stst, 2.98 in the Ti and 0.62 in the Ni group. A comparison of these two results shows that 48% of the dead cells were apoptotic in the NIM, 5.6% in the Stst 62% in the Ti and only 1.8% in the Ni group. The focal contacts were stained with a paxillin antibody and counted. There were marked differences in the number of focal Contacts Per unit area compared to NiTi (774 focal contacts): 335 in Stst (p less than or equal to0.01), 462 in Ti (p less than or equal to0.01) and 261 in Ni (p less than or equal to0.005). Out results show that NiTi is well tolerated by the osteoblastic type ROS-17 cells. (C) 2001 Elsevier Science Ltd. All rights reserved.