Syngeneic transplantation with peripheral blood mononuclear cells collected after the administration of recombinant human granulocyte colony-stimulating factor.
Syngeneic transplantation with peripheral blood mononuclear cells collected after the administration of recombinant human granulocyte colony-stimulating factor.
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DOI：
10.1182/blood.v82.7.1981.1981
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发表时间：
1993-10
期刊：
影响因子：
20.3
通讯作者：
Weaver Ch;Buckner Cd;K. Longin;F. Appelbaum;S. Rowley;K. Lilleby;J. Miser;R. Storb;J. Hansen;W. Bensinger
中科院分区：
文献类型：
--
作者：
Weaver Ch;Buckner Cd;K. Longin;F. Appelbaum;S. Rowley;K. Lilleby;J. Miser;R. Storb;J. Hansen;W. Bensinger
用重组人粒细胞集落刺激因子(rhG-CSF)给正常供者采集的未修饰外周血单个核细胞(PBMC)，在清髓治疗后的4例患者中进行了5次同基因移植。这四名正常捐赠者经历的唯一毒性是骨痛。4名患者接受了两次采集的PBMCs，一名患者接受了一次采集的第二次移植。中位数分别为20.53×10(8)总有核细胞/kg(20~25.5)，11.3×10(8)总单个核细胞/kg(6.52~17.2)，113.1×10(4)/kg CFU-GM(46.7~211.8)和9.6×10(6)CD34+细胞/kg(1.6~12.6)。中性粒细胞绝对值超过0.5x10(9)/L的中位时间为14天(10-18天)。恢复血小板输注的中位时间为11天(10~13天)。2例患者进行了外周血中CD3+T淋巴细胞的测定。平均每次采集3.04×10(10)个CD3+细胞。这比典型的骨髓移植中T淋巴细胞的数量增加了大约1个对数。重组人粒细胞集落刺激因子可用于动员正常供者外周血祖细胞，且毒性最小。使用注射rhG-CSF后收集的PBMC进行同种异体移植的研究，以确定永久移植物的能力以及移植物抗宿主病的发生率和严重程度是有必要的。
Five syngeneic transplants were performed in four patients following myeloablative therapy using unmodified peripheral blood mononuclear cells (PBMCs) collected after the administration of recombinant human granulocyte colony stimulating factor (rhG-CSF) to normal donors. The only toxicity experienced by the four normal donors was bone pain. Four patients received two collections of PBMCs, and a second transplant was performed in one patient with one collection. The patients received a median of 20.53 x 10(8) total nucleated cells/kg (range 20 to 25.5), 11.3 x 10(8) total mononuclear cells/kg (range 6.52 to 17.2), 113.1 x 10(4)/kg CFU-GM (range 46.7 to 211.8) and 9.6 x 10(6) CD34+ cells/kg (range 1.6 to 12.6) Post-transplant growth factors were not administered. The median time to an absolute neutrophil count greater than 0.5 x 10(9)/L was 14 days (range 10 to 18). The median time to platelet transfusion independence was 11 days (range 10 to 13). Two patients had the number of CD3+ T lymphocytes determined in the pheresis product. An average of 3.04 x 10(10) CD3+ cells were collected per pheresis. This represents an approximate 1 log increase over the number of T lymphocytes in a typical bone marrow transplant. Rh-GCSF can be used to mobilize peripheral blood progenitor cells from normal donors with minimal toxicity. Studies of allogeneic transplants using PBMCs collected after rhG-CSF administration to determine permanent grafting ability and the incidence and severity of graft-versus-host disease are warranted.