Differential susceptibility of different cell lines to swine-origin influenza A H1N1, seasonal human influenza A H1N1, and avian influenza A H5N1 viruses
Differential susceptibility of different cell lines to swine-origin influenza A H1N1, seasonal human influenza A H1N1, and avian influenza A H5N1 viruses
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DOI：
10.1016/j.jcv.2009.09.013
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发表时间：
2009-12-01
影响因子：
8.8
通讯作者：
Yuen, K. Y.
中科院分区：
文献类型：
--
作者：
Li, I. W. S.;Chan, K. H.;Yuen, K. Y.
背景：新型猪源甲型 H1N1 流感病毒（S-OIV）引起了当前的大流行。其组织向性和在不同细胞系中的复制尚不清楚。目的：比较S-OIV、季节性甲型H1N1（sH1N1）和禽流感甲型H5N1（H5N1）病毒感染的细胞系的生长特性以及温度对病毒复制的影响。研究设计：细胞病变效应（CPE）、抗原表达 对 S-OIV、sH1N1 和 H5N1 感染的 17 个细胞系进行免疫荧光 (IF) 和定量 RT-PCR 病毒载量分析。比较了它们在鸡胚中的复制效率。通过连续 5 天在 33°C、37°C 和 39°C 下测定温度对 Madin-Darby 犬肾 (MDCK) 细胞中病毒复制的影响。结果：S-OIV 在来自不同组织或器官和宿主物种的细胞系中复制，其病毒载量与 sH1N1 相当。在测试的 13 种人类细胞系中，Caco-2 的 S-OIV 病毒载量最高。 S-OIV 在猪肾细胞 PK-15 中表现出低病毒载量，没有 CPE 或抗原表达，H5N1 通过 CPE 和抗原表达表现出最多样化的细胞向性，并且在细胞系和尿囊液中表现出最高的病毒复制效率。所有三种病毒在 MDCK 细胞中均在 37 摄氏度下表现出最佳生长。结论：S-OIV、sH1N1 和 H5N1 的细胞系生长特征似乎在临床和病理学上与所涉及的解剖部位和严重程度相关。 PK-15 中 S-OIV 的低复制表明该病毒比猪更适合人类。 (C) 2009 Elsevier B.V. 保留所有权利。
Background: The novel swine-origin influenza A H1N1 virus (S-OIV) causes the current pandemic. Its tissue tropism and replication in different cell lines are not well understood.Objective: Compare the growth characteristics of cell lines infected by S-OIV, seasonal influenza A H1N1 (sH1N1) and avian influenza A H5N1 (H5N1) viruses and the effect of temperature on viral replication.Study design: Cytopathic effect (CPE), antigen expression by immunofluorescence (IF) and viral load profile by quantitative RT-PCR in 17 cell lines infected by S-OIV, sH1N1 and H5N1 were examined. Comparison of their replication efficiency in chick embryo was performed. The effect of temperature on viral replication in Madin-Darby canine kidney (MDCK) cells was determined by TCID50 at 33 degrees C, 37 degrees C and 39 degrees C for 5 consecutive days.Results: S-OIV replicated in cell lines derived from different tissues or organs and host species with comparable viral load to sH1N1. Among 13 human cell lines tested, Caco-2 has the highest viral load for S-OIV. S-OIV showed a low viral load with no CPE or antigen expression in pig kidney cell PK-15, H5N1 demonstrated the most diverse cell tropism by CPE and antigen expression, and the highest viral replication efficiency in both cell lines and allantoic fluid. All three viruses demonstrated best growth at 37 degrees C in MDCK cells.Conclusion: Cell line growth characteristics of S-OIV, sH1N1 and H5N1 appear to correlate clinically and pathologically with involved anatomical sites and severity. Low replication of S-OIV in PK-15 suggests that this virus is more adapted to human than swine. (C) 2009 Elsevier B. V. All rights reserved.