Viral expression in two myelomonocytic cell lines obtained from long-term bone marrow culture infected with the Friend polycythemia-inducing virus (FV-P).
Viral expression in two myelomonocytic cell lines obtained from long-term bone marrow culture infected with the Friend polycythemia-inducing virus (FV-P).
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从感染 Friend 红细胞增多症诱导病毒 (FV-P) 的长期骨髓培养中获得的两种骨髓单核细胞系中的病毒表达。
DOI：
10.1016/0042-6822(82)90065-4
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发表时间：
1982
期刊：
影响因子：
3.7
通讯作者：
C. Larsen
中科院分区：
文献类型：
--
作者：
D. Mathieu;J. Heard;S. Fichelson;S. Gisselbrecht;B. Sola;C. Larsen
对长期感染Friend多核细胞增多症病毒的小鼠骨髓细胞亚系317和G-31的病毒释放和病毒蛋白表达进行了研究。317细胞系永久产生具有生物活性的脾病灶形成病毒(SFFV)，并表达gp52SFFV，该基因已被描述为SFFV基因组编码的。相比之下，G-31细胞株虽然释放了Friend Helper白血病病毒(FMuLV)，但没有任何SFFV表达。这两株细胞都表达了一种新的gp70相关糖蛋白，表观分子量为66,000。该gp66菌株在代谢上不稳定，既不被包装成病毒颗粒，也不能在培养基中释放。它能被抗Rauscher gp70抗血清识别，但不能被抗MCF gp70或抗p15E抗血清识别。在红系Friend肿瘤细胞系、317和G-31系释放病毒感染的成纤维细胞、非粒单核细胞感染的造血细胞中均未检测到gp66。然而，在活体和体外建立的单核细胞来源的细胞系中经常观察到它。因此，gp66是一种F-MuLV gp70相关产物，与髓系特异性连锁。
Two subclones of monomyelocytic cell lines (317 and G-31), issued from mouse long-term bone marrow cultures infected with Friend polycythemia-inducing virus, were studied for virus release and viral protein expression. The 317 cell line permanently produced biologically active spleen focus-forming virus (SFFV) and expressed the gp52SFFVwhich has been described as encoded by the SFFV genome. In contrast, the G-31 cell line was devoid of any SFFV expression although it released Friend helper leukemia virus (FMuLV). Both lines expressed a new gp70-related glycoprotein with an apparent molecular weight of 66,000. This gp66 species was metabolically unstable and was not packaged into viral particles nor released in culture medium. It was recognized by an anti-Rauscher gp70 antiserum but not by anti-MCF gp70 or anti-p15E antisera. gp66 was not found in erythroblastic Friend tumor cell lines, in fibroblasts infected with virus released by lines 317 and G-31, or in nonmyelomonocytic infected hematopoietic cells. However, it was constantly observed in cell lines with a myelomonocytic origin establishedin vivoandin vitro. Therefore, gp66 is a F-MuLV gp70-related product specifically linked to the myeloid lineage.