Multilocus sequence typing: A portable approach to the identification of clones within populations of pathogenic microorganisms
Multilocus sequence typing: A portable approach to the identification of clones within populations of pathogenic microorganisms
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DOI：
10.1073/pnas.95.6.3140
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发表时间：
1998-03-17
影响因子：
11.1
通讯作者：
Spratt, BG
中科院分区：
文献类型：
--
作者：
Maiden, MCJ;Bygraves, JA;Spratt, BG
用于病原微生物表征的传统和分子分型方案的便携性较差，因为它们指示难以在实验室之间进行比较的变异。为了克服这些问题，我们提出了多位点序列分型（MLST），其利用核苷酸序列数据的明确性质和电子可移植性来表征微生物。为了评估MLST，我们确定了来自侵袭性疾病和健康携带者的脑膜炎奈瑟菌的107个分离株的参考组中的11个管家基因的约470-bp片段的序列。对于每个位点，将等位基因分配为任意数目，并通过聚类分析从多位点等位基因谱的成对差异构建树状图。获得的菌株协会是一致的克隆分组先前确定的多位点酶电泳。选择保留通过使用所有11个基因座实现的分辨率和一致性的6个基因片段的子集。来自血清群A、B和C脑膜炎球菌的超毒力谱系的大多数分离物对于所有基因座是相同的，或者仅在单个基因座处与大多数类型不同，因此使用6个基因座的MLST可靠地鉴定了与侵袭性疾病相关的主要脑膜炎球菌谱系，MLST可以应用于几乎所有的细菌物种和其他单倍体生物，包括那些难以培养的。MLST相对于其他分子分型方法的压倒性优势在于，序列数据在实验室之间是真正可移植的，允许每个物种的一个扩展的全球数据库被放置在万维网网站上，从而使全球流行病学的分子分型数据能够通过互联网进行交换。
Traditional and molecular typing schemes for the characterization of pathogenic microorganisms are poorly portable because they index variation that is difficult to compare among laboratories. To overcome these problems, we propose multilocus sequence typing (MLST), which exploits the unambiguous nature and electronic portability of nucleotide sequence data for the characterization of microorganisms, To evaluate MLST, we determined the sequences of approximate to 470-bp fragments from 11 housekeeping genes in a reference set of 107 isolates of Neisseria meningitidis from invasive disease and healthy carriers, For each locus, alleles were assigned arbitrary numbers and dendrograms were constructed from the pairwise differences in multilocus allelic profiles by cluster analysis. The strain associations obtained were consistent with clonal groupings previously determined by multilocus enzyme electrophoresis. A subset of six gene fragments was chosen that retained the resolution and congruence achieved by using all 11 loci, Most isolates from hyper-virulent lineages of serogroups A, B, and C meningococci were identical for all loci or differed from the majority type at only a single locus, MLST using six loci therefore reliably identified the major meningococcal lineages associated with invasive disease, MLST can be applied to almost all bacterial species and other haploid organisms, including those that are difficult to cultivate. The overwhelming advantage of MLST over other molecular typing methods is that sequence data are truly portable between laboratories, permitting one expanding global database per species to be placed on a World-Wide Web site, thus enabling exchange of molecular typing data for global epidemiology via the Internet.