Light-Driven Activation of RNA-Guided Nucleic Acid Cleavage
Light-Driven Activation of RNA-Guided Nucleic Acid Cleavage
复制标题
RNA 引导的核酸切割的光驱动激活
DOI：
10.1021/acschembio.0c00105
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发表时间：
2020-06-19
影响因子：
4
通讯作者：
Zhou, Xiang
中科院分区：
文献类型：
--
作者：
Wang, Shaoru;Wei, Lai;Zhou, Xiang
作为最有利的刺激之一，光活化提供了一种有利的操纵生物对象的方法。在当前的研究中，我们成功证明了光激活指南RNA（GRNA）策略来控制CRISPR系统。通过结合光片保护组，CRISPR函数变得很少，但是将酰化的GRNA暴露于365 nm的光线下，触发了掩盖组的去除，从而挽救了CRISPR功能。此外，我们的策略已成功用于控制人类细胞中的基因编辑。因此，这项概念证明的研究证明了我们策略对化学生物学应用多功能应用的潜力。
As one of the most favorable stimuli, photoactivation provides an advantageous way to manipulate biological objects. In the current study, we have successfully demonstrated the use of light activation guide RNA (gRNA) strategy for controlling CRISPR systems. By conjugating photolabile protecting groups, the CRISPR functions became minimal, but exposure of acylated gRNAs to 365 nm light triggers the removal of masking groups, leading to the rescue of CRISPR functions. Furthermore, our strategy has been successfully used to control gene editing in human cells. This proof-of-concept study therefore demonstrates the promising potential of our strategy to versatile applications in chemical biology.