IgG4 and IgE are the major immunoglobulins targeting the NC16A domain of BP180 in bullous pemphigoid:: Serum levels of these immunoglobulins reflect disease activity
IgG4 and IgE are the major immunoglobulins targeting the NC16A domain of BP180 in bullous pemphigoid:: Serum levels of these immunoglobulins reflect disease activity
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DOI：
10.1067/mjd.2000.103986
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发表时间：
2000-04-01
影响因子：
13.8
通讯作者：
Zillikens, D
中科院分区：
文献类型：
--
作者：
Döpp, R;Schmidt, E;Zillikens, D
背景：大疱性类天疱疮（BP）是一种自身免疫性水疱性疾病，与抗半桥粒糖蛋白BP 180的自身抗体有关。BP 180的非胶原（NC）16 A结构域最近被证明含有BP血清识别的主要抗原位点。目的：本研究的目的是表征BP患者治疗前和治疗过程中循环中存在的针对BP 180 NC 16 A的自身抗体的亚类分布和精细特异性。通过免疫印迹和酶联免疫吸附试验分析了18份BP血清中是否存在与BP 180 NC 16 A结构域上的各个位点反应的IgG 1、IgG 2、IgG 3、IgG 4和IgE。在治疗开始前和治疗开始后4周和8周的时间点收集血清。结果：我们鉴定了IgG 4和IgE作为优先与BP 180 NC 16 A内的两个不同表位（MCW-1和MCW-2）反应的主要免疫球蛋白。这些自身抗体的水平与BP的疾病活动相关。在疾病的过程中，没有观察到变化方面的免疫球蛋白亚类主要与BP 180 NC 1GA或在此domain.Conclusion的特定抗原表位反应：我们的数据表明，缓解BP是通过降低血清IgE水平和不同的IgG亚类与BP 180 NC 16 A反应。
Background: Bullous pemphigoid (BP) is an autoimmune blistering disease associated with autoantibodies against the hemidesmosomal glycoprotein BP180. The noncollagenous (NC)16A domain of BP180 has recently been shown to harbor major antigenic sites recognized by BP sera.Objective: The purpose of this study was to characterize the subclass distribution and fine specificities of autoantibodies to BP180 NC16A present in the circulation of patients with BP before, and during the course of, therapy for this disease.Methods: Eighteen BP sera were analyzed by immunoblotting and enzyme-linked immunosorbent assay for the presence of IgG1, IgC2, IgG3, IgG4, and IgE reactive with various sites on the BP180 NC16A domain. The sera were collected before treatment was started and at 4- and 8-week time points after initiation of treatment.Results: We identified IgG4 and IgE as the major immunoglobulins that preferentially react with two distinct epitopes (MCW-1 and MCW-2) within BP180 NC16A. Levels of these autoantibodies correlated with disease activity in BP. During the course of disease, no change was observed with regard to the immunoglobulin subclass predominantly reacting with BP180 NC1GA or the specific epitopes within this domain.Conclusion: Our data demonstrate that remission of BP is paralleled by a decrease of serum levels of IgE and the different IgG subclasses reactive with BP180 NC16A.