Thermodynamic analysis of the kinetics reactions of the production of FAME and FAEE using Novozyme 4
Thermodynamic analysis of the kinetics reactions of the production of FAME and FAEE using Novozyme 4
复制标题
DOI：
10.1016/j.fuproc.2010.12.023
复制
发表时间：
2011-05
期刊：
影响因子：
--
通讯作者：
Astria D. Ferrão-Gonzales;I. C. Véras;F. A. Silva;H. Alvarez;Vitor Hugo Moreau
中科院分区：
文献类型：
--
作者：
Astria D. Ferrão-Gonzales;I. C. Véras;F. A. Silva;H. Alvarez;Vitor Hugo Moreau
生物柴油是一种有望成为石油柴油替代品的生物燃料。酶催化法是生产生物柴油最有前途的技术之一。然而，所使用的大多数酶的催化性能较低，使得这种过程既昂贵又耗时。以固定化南极洲假丝酵母脂肪酶(Novozym 435)为催化剂，研究了不同温度下甲醇和乙醇解生物柴油的酶促反应动力学。用气相色谱对反应动力学进行跟踪，并用Arrhenius方程对数据进行了热力学分析。结果表明，在13℃以上的温度下，甲醇的反应速度要快于乙烷的反应速度，反应动力学的热力学分析表明，由于反应的正活化熵变化，甲醇更适合作为酰基受体。这些数据可能对酶法生产生物柴油的新酶和新工艺的开发有用。
Biodiesel is a biofuel expected to become a substitute for petroleum diesel. One of the most promising technologies for production of biodiesel is enzymatic catalysis. However, low catalytic performance of most of the enzymes employed makes such processes expensive and time-consuming. This work describes a kinetic study of the enzymatic production of biodiesel at different temperatures using either methanolysis or ethanolysis, using immobilized lipase from Candida antarctica (Novozym 435) as catalyst. Reactions kinetics were followed by GC, and data were used to perform thermodynamic analysis of the transition state using Arrhenius equation. We found that methanolysis is faster than ethanolysis at temperatures above 13°C. Thermodynamic analysis of the kinetics of reactions showed that methanol is favored as acyl acceptor due to the positive activation entropy change of reaction. These data may be useful in the development of new enzymes and new processes for enzymatic production of biodiesel.