Wheat germ agglutinin‐apoHRP gold: A new retrograde tracer for light‐ and electron‐microscopic single‐ and double‐label studies
Wheat germ agglutinin‐apoHRP gold: A new retrograde tracer for light‐ and electron‐microscopic single‐ and double‐label studies
复制标题
小麦胚芽凝集素-apoHRP 金:用于光镜和电子显微镜单标记和双标记研究的新型逆行示踪剂
DOI:
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发表时间:
1987
期刊:
影响因子:
--
通讯作者:
D. Menétrey
中科院分区:
文献类型:
--
作者:
A. Basbaum;D. Menétrey
In this report, we describe a new colloidal‐gold‐labelled retrograde tracer, wheat germ agglutinin (WGA) conjugated to enzymatically inactive horseradish peroxidase (apoHRP). This protein gold complex (WGAapoHRP‐Au) is a sensitive marker for retrograde tracing of the projections of CNS neurons at the light‐microscopic (LM) level when a silver‐enhancement procedure is used to detect the gold in the tracer. For electron‐microscopic (EM) analysis, the silver‐enhanced sections undergo a further gold‐toning step. This protects against rapid oxidation and dissolution of the silver precipitate during the osmication procedure.
影响因子:
3.2
作者:
RYE, DB;SAPER, CB;WAINER, BH
通讯作者:
WAINER, BH
DOI:
10.1177/34.8.2426333
发表时间:
1986
期刊:
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子:
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作者:
Lakos,S;Basbaum,AI
通讯作者:
Basbaum,AI
DOI:
10.1177/30.8.6181123
发表时间:
1982
期刊:
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子:
--
作者:
Bowker,RM;Westlund,KN;Sullivan,MC;Coulter,JD
通讯作者:
Coulter,JD