Arabidopsis thaliana 26S Proteasome Subunits RPT2a and RPT5a Are Crucial for Zinc Deficiency-Tolerance
Arabidopsis thaliana 26S Proteasome Subunits RPT2a and RPT5a Are Crucial for Zinc Deficiency-Tolerance
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DOI：
10.1271/bbb.100794
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发表时间：
2011-03
期刊：
影响因子：
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通讯作者：
Takuya Sakamoto;Takehiro Kamiya;Kaori Sako;J. Yamaguchi;Mutsumi Yamagami;T. Fujiwara
中科院分区：
文献类型：
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作者：
Takuya Sakamoto;Takehiro Kamiya;Kaori Sako;J. Yamaguchi;Mutsumi Yamagami;T. Fujiwara
RPTs（regulatory particle triple-A-ATPase）是26 S蛋白酶体的组成部分。我们发现RPT 2a和RPT 5a在缺锌耐受中的新作用。在RPT 2a和RPT 5a中携带T-DNA的拟南芥突变体比野生型对锌缺乏更敏感。在rpt突变体中，地上部锌含量与野生型相似。锌缺乏诱导基因的转录本在rpt突变体中高度积累，表明rpt突变体患有各种锌缺乏症状，尽管锌水平没有降低。脂质过氧化水平，已知锌缺乏下增加，在rpt突变体比野生型。聚泛素化蛋白质积累后暴露于锌缺乏症，特别是在rpt突变体。总体而言，这项研究表明，RPT 2a和RPT 5a参与锌缺乏耐受性，可能通过减轻氧化应激和/或加工多聚泛素化蛋白。
RPTs (regulatory particle triple-A-ATPase) are components of 26S proteasome. We found novel roles of RPT2a and RPT5a in Zn deficiency-tolerance. Arabidopsis thaliana mutants carrying T-DNA in RPT2a and RPT5a were more sensitive to Zn deficiency than the wild-type. In the rpt mutants, the shoot Zn contents were similar to those of the wild-type. Transcripts of Zn deficiency-inducible genes were highly accumulated in the rpt mutants, suggesting that the rpt mutants suffer from various Zn deficiency symptoms, although the Zn levels are not reduced. Lipid peroxidation levels, known to be increased under Zn deficiency, were higher in the rpt mutants than in the wild-type. Poly-ubiquitinated proteins were accumulated upon exposure to Zn deficiency, especially in the rpt mutants. Overall, this study indicates that RPT2a and RPT5a are involved in Zn deficiency-tolerance, possibly through alleviation of oxidative stresses and/or processing of poly-ubiquitinated proteins.