Different isolates from Leishmania braziliensis complex induce distinct histopathological features in a murine model of infection.
Different isolates from Leishmania braziliensis complex induce distinct histopathological features in a murine model of infection.
复制标题
巴西利什曼原虫复合体的不同分离株在小鼠感染模型中诱导出不同的组织病理学特征。
DOI：
10.1016/j.vetpar.2009.07.019
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发表时间：
2009
影响因子：
2.6
通讯作者：
M. A. Souza
中科院分区：
文献类型：
--
作者：
C. G. Pereira;A. L. N. Silva;Patrícia de Castilhos;E. Mastrantonio;Richard A. Souza;R. Romão;Renata J Rezende;J. Pena;M. E. Beletti;M. A. Souza
The aim of this study was to evaluate the histopathological features in tissues of mice infected by human isolates (I, II, and III) or the reference M2903 strain of Leishmania braziliensis complex. BALB/c and C57Bl/6 mice were infected in the hind footpad with 106stationary-phase promastigotes of L. braziliensis complex. The evolution of lesions was observed for 10 weeks and the animals were then euthanized and liver, spleen and popliteal lymph nodes were collected. Tissues were stained with hematoxylin and eosin and analyzed by immunohistochemistry assay. Increased thickness of infected footpads was observed in all animals, lesions were nodular and non-ulcerated. Mice infected with isolate I presented inflammatory infiltrates consisting predominantly of mononuclear cells in all tissues examined, and also a great number of megakaryocytes, compared with other isolates. Infection with isolate II led to an infected footpad enlargement not seen in other isolates. In addition, mononuclear infiltrates in the liver and hemosiderin in spleen were noted. Conversely, mice infected with either isolate III or M2903 strain only showed an increased number of megakaryocytes in spleen. All tissues examined had detectable amastigote forms of Leishmania by immunohistochemistry in all groups. Taking together, our results showed an unforeseen behavior of different isolates of L. braziliensis complex that led to diverse pathological findings.
实验性内脏利什曼病的获得性耐药和肉芽肿形成。
DOI：
--
发表时间：
1992
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Murray,HW;Squires,KE;Miralles,CD;Stoeckle,MY;Granger,AM;Granelli-Piperno,A;Bogdan,C
通讯作者：
Bogdan,C