THE HEMIDESMOSOMAL PLAQUE .1. CHARACTERIZATION OF A MAJOR CONSTITUENT PROTEIN AS A DIFFERENTIATION MARKER FOR CERTAIN FORMS OF EPITHELIA
THE HEMIDESMOSOMAL PLAQUE .1. CHARACTERIZATION OF A MAJOR CONSTITUENT PROTEIN AS A DIFFERENTIATION MARKER FOR CERTAIN FORMS OF EPITHELIA
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DOI：
10.1111/j.1432-0436.1990.tb00475.x
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发表时间：
1990-12-01
期刊：
影响因子：
2.9
通讯作者：
FRANKE, WW
中科院分区：
文献类型：
--
作者：
OWARIBE, K;KARTENBECK, J;FRANKE, WW
为了检查半桥粒结构的组成蛋白是否可以用作上皮分化某些途径的标记，我们检查了主要的 M(r)-大约 230 000 斑块蛋白，即“大疱性类天疱疮”(BP) 抗原的出现。在免疫细胞化学和免疫印迹中使用不同的人自身抗体制剂检查了几种牛、大鼠和人体组织以及牛细胞培养物系。我们报告说，这种蛋白质也通过表达文库的 cDNA 克隆和 DNA 测序明确鉴定出来，不仅存在于不同的复层上皮中，而且显然总是存在于半桥粒结构中、膀胱尿路上皮以及气管、支气管和几个腺体的复杂上皮中，特别是含有肌上皮的皮肤腺、乳腺和唾液腺中。然而，在所有单层上皮细胞和据报道具有与半桥粒结构相似的质膜下密度的一些组织中，例如心脏、脑膜和神经束膜的浦肯野纤维，该蛋白质不存在。乳腺来源的上皮细胞系 (BMGE + H) 富含半桥粒。这已用于研究在用分散酶（一种蛋白水解酶）处理期间细胞片分离时，含有半桥粒的质膜结构域进入细胞质囊泡的内吞作用。我们建议在胎儿和肿瘤发育研究（包括癌症的分化诊断）中使用 M(r)- 大约 230 000 斑块蛋白作为上皮细胞类型和上皮源性肿瘤的某些子集的选择性标记。
To examine whether constituent proteins of hemidesmosomal structures can be used as markers for certain pathways of epithelial differentiation we have examined the occurrence of the major M(r)- approximately 230 000 plaque protein, the "bullous pemphigoid" (BP) antigen. Several bovine, rat and human tissues and bovine cell culture lines were examined, using different human autoantibody preparations in immunocytochemistry and immunoblotting. We report that this protein, also unequivocally identified by cDNA cloning from expression libraries and DNA sequencing, occurs not only in different stratified epithelia but also, apparently always in hemidesmosomal structures, in urothelium of bladder and the complex epithelia of trachea, bronchus and several glands, notably myoepithelium-containing skin glands, the mammary gland and salivary glands. The protein is absent, however, in all single-layered epithelia and in several tissues reported to have subplasmalemmal densities structurally similar to hemidesmosomes, such as Purkinje fibers of heart, meninges and perineuria. A mammary-gland-derived epithelial cell line (BMGE + H) is particularly rich in hemidesmosomes. This has been used to study the endocytotic uptake of hemidesmosome-containing plasma membrane domains into cytoplasmic vesicles upon detachment of cell sheets during treatment with dispase, a proteolytic enzyme. We propose to use the M(r)- approximately 230 000 plaque protein as a marker selective for certain subsets of epithelial cell types and epithelium-derived tumors in studies of fetal and tumor development, including differentiation diagnosis of carcinomas.