Quality Evaluation of Polar and Active Components in Crude and Processed Fructus Corni by Quantitative Analysis of Multicomponents with Single Marker.
Quality Evaluation of Polar and Active Components in Crude and Processed Fructus Corni by Quantitative Analysis of Multicomponents with Single Marker.
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DOI：
10.1155/2016/6496840
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发表时间：
2016
影响因子：
2.6
通讯作者：
Liu Z
中科院分区：
文献类型：
--
作者：
Jiang Y;Chen H;Wang L;Zou J;Zheng X;Liu Z
Objective.建立一种多组分单标记定量分析法（QAMS），用于山茱萸中极性活性成分的同时测定。方法.以马钱苷为内标，建立了没食子酸、5-羟甲基-2-糠醛、莫诺苷、当药苷、山茱萸苷、7α-O-甲基莫诺苷、7β-O-甲基莫诺苷、7α-O-乙基莫诺苷、7β-O-乙基莫诺苷和山茱萸苷的相对校正因子。根据各组分的相对循环系数分别计算各组分的含量。用外标法计算了11种成分的含量，并与QAMS法进行了比较。结果测定了21种山茱萸生品和10种山茱萸炮制品中11种成分的含量。结果表明，QAMS法与外标法的定量结果无显著性差异。结论QAMS法可作为山茱萸及其炮制品中极性成分和活性成分的准确、简便的测定方法。
Objective. To develop a quantitative analysis of multicomponents by single-marker (QAMS) method for the simultaneous determination of polar active components in Fructus Corni. Methods. Loganin was selected as the internal reference, and the relative correction factors (RCFs) of gallic acid, 5-hydroxymethyl-2-furfural, morroniside, sweroside, cornin, 7α-O-methylmorroniside, 7β-O-methylmorroniside, 7α-O-ethylmorroniside, 7β-O-ethylmorroniside, and cornuside were established. The contents of multicomponents were then calculated based on their RCFs, respectively. Contents of the 11 components were also calculated by external standard method and compared with those of the QAMS method. Results. The contents of the 11 components in 21 crude and 10 processed Fructus Corni products were measured. No significant difference was found in the quantitative results of the QAMS and external standard methods. Conclusion. QAMS could serve as an accurate and convenient method in determining the polar and active components in Fructus Corni and its processed products.