Deep sequencing reveals global patterns of mRNA recruitment during translation initiation.
Deep sequencing reveals global patterns of mRNA recruitment during translation initiation.
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深度测序揭示翻译起始过程中 mRNA 募集的全局模式
DOI：
10.1038/srep30170
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发表时间：
2016-07-27
影响因子：
4.6
通讯作者：
Su XD
中科院分区：
文献类型：
--
作者：
Gao R;Yu K;Nie J;Lian T;Jin J;Liljas A;Su XD
在这项工作中，我们开发了一种方法来系统地研究翻译启动过程中mRNA的序列偏好。传统上，已经在单分子水平上研究了翻译启动的动态过程，并具有有限的测序可能性。使用深层测序技术，我们确定了起始复合物不同阶段的序列偏好。我们的结果为翻译起始区域（TIR）中的起始元素（包括S1结合序列，Shine-Dalgarno（SD）/抗SD相互作用和第二个密码子）提供了全面而动态的视图。此外，我们的实验揭示了在mRNA募集的整个动态过程中的构象变化和区域动力学。
In this work, we developed a method to systematically study the sequence preference of mRNAs during translation initiation. Traditionally, the dynamic process of translation initiation has been studied at the single molecule level with limited sequencing possibility. Using deep sequencing techniques, we identified the sequence preference at different stages of the initiation complexes. Our results provide a comprehensive and dynamic view of the initiation elements in the translation initiation region (TIR), including the S1 binding sequence, the Shine-Dalgarno (SD)/anti-SD interaction and the second codon, at the equilibrium of different initiation complexes. Moreover, our experiments reveal the conformational changes and regional dynamics throughout the dynamic process of mRNA recruitment.