The incidence of Helicobacter pylori acquisition in children of a Canadian First Nations community and the potential for parent-to-child transmission.
The incidence of Helicobacter pylori acquisition in children of a Canadian First Nations community and the potential for parent-to-child transmission.
复制标题
加拿大原住民社区儿童感染幽门螺杆菌的发生率以及亲子传播的可能性。
DOI：
10.1111/j.1083-4389.2004.00199.x
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发表时间：
2004
期刊：
影响因子：
4.4
通讯作者：
Bernstein,CharlesN
中科院分区：
文献类型：
--
作者：
Sinha,SamirK;Martin,Bruce;Gold,BenjaminD;Song,Qunsheng;Sargent,Michael;Bernstein,CharlesN
背景和目的：我们以前曾报道过，Wasagamack是加拿大的一个原住民社区，其幽门螺杆菌的血清阳性率为95%，0 - 12岁儿童的粪便抗原检测阳性率为56%。我们的目的是确定感染率收购和可能的方式传播的childhoodHelicobacter pylori infection in this Canadian First Nations community. Methods.儿童谁是以前阴性为幽门螺杆菌粪便抗原检测在1999年8月有资格在2000年8月登记; 50例（77%）合格儿童接受粪便采集。使用Premier Platinum HpSA检测法检测幽门螺杆菌粪便抗原状态。通过三种互补的幽门螺杆菌特异性PCR检测法对饮用水样本、母亲唾液、母乳、当地浆果和苍蝇进行检测。安抚奶嘴或奶瓶奶嘴，收集了16名儿童。结果：50例患儿粪便中幽门螺杆菌阳性率为16%（8/50），阳性率为100%（8/50）。五个没有其他兄弟姐妹感染，三个有感染的兄弟姐妹。所有受感染儿童的母亲都是幽门螺杆菌阳性。新感染儿童的中位年龄为6岁（范围1 - 13岁）。经PCR检测，78%（18/23）的母亲唾液样本、69%（11/16）的安抚奶嘴水样本和9%（1/11）的感染家庭水样本阳性。所有24个PCR产物的DNA片段测序显示与ATCC型菌株H.pyloris.Conclusions的同源性为99%，儿童期H.pylorisacidity的发生率为16%，超过1年，并不依赖于兄弟姐妹感染的数量。在唾液和抚慰水中发现同源的幽门螺杆菌DNA表明人传人的可能性，特别是通过口-口途径。因此，有可能在该人群和其他流行社区中进行进一步调查，以预防通过这种方式传播感染。
Background and Aims.We have previously reported that Wasagamack, a Canadian First Nations community has a seroprevalence rate ofHelicobacter pyloriof 95% and a prevalence rate among children aged 0–12 years as measured by stool antigen testing of 56%. We aimed to determine the rate of infection acquisition and possible modes of transmission of childhoodHelicobacter pyloriinfection in this Canadian First Nations community.Methods.Children who were previously negative forH. pyloriby stool antigen testing in August 1999 were eligible for enrollment in August 2000; 50 (77%) eligible children underwent stool collection.H. pyloristool antigen status was tested using the Premier Platinum HpSA test. Drinking water samples, maternal saliva, breast milk, local berries and flies were tested by three complementaryH. pylori‐specific PCR assays. Soothers or bottle nipples, collected from 16 children whoseH. pyloristool antigen status was determined, were bathed in sterile water and this water was tested by PCR.Results.Stool was positive forH. pyloriin 16% (8/50) of children retested. Five had no other siblings infected and three had infected siblings. The mothers of all children infected were positive forH. pylori. The median age of newly infected children was 6 years (range 1–13 years). By PCR, 78% (18/23) mothers’ saliva samples, 69% (11/16) soother water samples and 9% (1/11) water samples from infected homes tested positive. All of 24 sequenced PCR‐produced DNA fragments from samples showed 99% homology with that from ATCC type strainH. pylori.Conclusions.The rate of childhoodH. pyloriacquisition was 16% over 1 year, and was not dependent on number of siblings infected. The finding of homologousH. pyloriDNA in saliva and in soother water suggests the possibility of human to human transmission, particularly via an oral–oral route. Thus, there is the potential for further investigations in this population and other endemic communities that are directed at prevention of infection transmission via this modality.