Unique mitogenomic features in the scleractinian family Pocilloporidae (Scleractinia: Astrocoeniina)
Unique mitogenomic features in the scleractinian family Pocilloporidae (Scleractinia: Astrocoeniina)
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DOI：
10.1007/s10126-008-9093-x
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发表时间：
2008-09-01
影响因子：
3
通讯作者：
Chen, Chaolun Allen
中科院分区：
文献类型：
--
作者：
Chen, Chienhsun;Chiou, Chih-Yung;Chen, Chaolun Allen
从石珊瑚Stylophora pistillata，Pocillopora damicornis和Madracis mirabilis中获得了三种线粒体（mt）基因组的完整DNA序列，并与已发表的mt基因组进行了比较，以阐明Pocilloporidae家族的遗传学独特特征。结果表明，小孔珊瑚线粒体基因组全长为16951 ~ 17425 bp，有义链A+T含量为68.4%~ 70.2%。蛋白质编码基因的基因顺序与其他石珊瑚相同。新的atp 8基因，首次在同源Seriatopora珊瑚，也证实了使用逆转录聚合酶链反应（RT-PCR），北方印迹，和序列分析在其他属的Pocilloporidae。atp 6和nad 4之间的基因间隔区，包含不同的重复元件，保守的序列块和结构域，以及功能结构，具有典型的特征，推定的控制区的四个珊瑚属。一个重复的trnW，检测到在该地区接近cox 1基因，并分享其原始对应的高度保守的一级和二级结构，被发现在Seriatopora和Stylophora。这些分子特征是独特的，并提供了系统发育信息，为未来的评估家庭Pocilloporidae的地位，在石珊瑚的进化历史。
The complete DNA sequences of three mitochondrial (mt) genomes were obtained from the scleractinian corals, Stylophora pistillata, Pocillopora damicornis, and Madracis mirabilis, and were compared to the published mt genomes to elucidate phylogenetically unique features of the family Pocilloporidae. The entire mt genomes of pocilloporid corals ranged from 16,951 to 17,425 bp with the A+T contents of their sense strands ranging from 68.4% to 70.2%. The gene order of protein-coding genes was identical to those of other scleractinian corals. The novel atp8 gene, first described in confamilial Seriatopora corals, was also confirmed using reverse transcription-polymerase chain reaction (RT-PCR), Northern blot, and sequence analyses in other genera of the Pocilloporidae. The intergenic spacer between atp6 and nad4, containing distinct repeated elements, conserved sequence blocks and domains, and functional structures, possesses typical characteristics of a putative control region for the four coral genera. A duplicated trnW, detected in the region close to the cox1 gene and which shares the highly conserved primary and secondary structures of its original counterpart, was discovered in both Seriatopora and Stylophora. These molecular characteristics are unique and provide the phylogenetic information for future evaluation of the status of the family Pocilloporidae in the evolutionary history of scleractinian corals.