Antioxidant activity and ultra-performance LC-electrospray ionization-quadrupole time-of-flight mass spectrometry for phenolics-based fingerprinting of Rose species: Rosa damascena, Rosa bourboniana and Rosa brunonii
Antioxidant activity and ultra-performance LC-electrospray ionization-quadrupole time-of-flight mass spectrometry for phenolics-based fingerprinting of Rose species: Rosa damascena, Rosa bourboniana and Rosa brunonii
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DOI：
10.1016/j.fct.2008.11.036
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发表时间：
2009-02-01
影响因子：
4.3
通讯作者：
Bari, Shamsher S.
中科院分区：
文献类型：
--
作者：
Kumar, Neeraj;Bhandari, Pamita;Bari, Shamsher S.
玫瑰是最重要的观赏植物之一，其果实和花朵用于各种食品，营养产品和不同的传统药物。采用1，1-二苯基-2-苦基肼基（DPPH）自由基法，研究了3种月季（蔷薇科蔷薇属蔷薇通过溶液的变色来测量清除DPPH自由基的能力。采用甲醇提取法对R. brunonii具有最大的自由基清除活性（64.5 ± 0.38%），其次是R. bourboniana（51.8 ± 0.46%）和R.在100 μ g/ml时，阿米卡星（43.6 +/- 0.25%）。同时，采用超高效液相色谱-电喷雾四极杆飞行时间质谱联用技术（UPLC-ESI-QTOF-MS）对月季鲜花甲醇提取物中的酚类成分进行了研究。采用直接进样-电喷雾定量飞行时间串联质谱（ESI-QTOF-MS/MS）负离子模式对酚类成分进行了研究。特征电喷雾串联质谱（ESI-MS/MS）光谱与其他诊断碎片离子产生的逆向Diels-Alder（RDA）裂解途径的黄酮类化合物被记录。多酚类化合物在三种植物中的相对分布具有明显的相似性。槲皮素、山奈酚及其苷类在三种植物中均占优势。(C)2008爱思唯尔有限公司保留所有权利。
Roses are one of the most important groups of ornamental plants and their fruits and flowers are used in a wide variety of food, nutritional products and different traditional medicines. The antioxidant activity of methanolic extracts from fresh flowers of three rose species (Rosa damascena, Rosa bourboniana and Rosa brunonii) was evaluated by 1,1-diphenyl-2-picryl hydrazyl (DPPH) free-radical method. The ability to scavenge DPPH radical was measured by the discoloration of the solution. The methanolic extract from R. brunonii exhibited maximum free-radical-scavenging activity (64.5 +/- 0.38%) followed by R. bourboniana (51.8 +/- 0.46%) and R. damascena (43.6 +/- 0.25%) at 100 mu g/ml. Simultaneously, ultra-performance liquid chromatography coupled with electrospray ionization-quadrupole time-of-flight mass spectrometry (UPLC-ESI-QTOF-MS) was used to study phenolic composition in the methanolic extracts from the fresh flowers of rose species. The phenolic constituents were further investigated by direct infusion-ESI-QTOF-MS/MS in negative ion mode. Characteristic Electrospray ionization tandem mass spectrometry (ESI-MS/MS) spectra with other diagnostic fragment ions generated by retro Diels-Alder (RDA) fragmentation pathways were recorded for the flavonoids. Distinct similarities were observed in the relative distribution of polyphenolic compounds among the three species. The dominance of quercetin, kaempferol and their glycosides was observed in all the three species. (C) 2008 Elsevier Ltd. All rights reserved.