Identification of forensically relevant body fluids using a panel of differentially expressed microRNAs
Identification of forensically relevant body fluids using a panel of differentially expressed microRNAs
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DOI：
10.1016/j.fsigss.2009.08.184
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发表时间：
2009-12-01
期刊：
影响因子：
--
通讯作者：
Ballantyne, J.
中科院分区：
文献类型：
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作者：
Hanson, E.;Lubenow, H.;Ballantyne, J.
大量研究表明，使用信使 RNA (mRNA) 分析能够识别法医生物染色剂的体液来源。然而，这些测定中使用的放大器的大小可能不适合用于法医案件工作中经常遇到的降解或受损的样品。因此，开发了一种通过使用 microRNA (miRNA) 分析来识别体液的新方法。 MicroRNA 长度仅为 20-25 个碱基，可能更适合用于降解或环境受损的生物样品。我们已经鉴定出一组九个差异表达的 miRNA，允许使用少至 50 pg 的总 RNA 来鉴定法医生物染色剂的体液来源。 (C) 2009 Elsevier Ireland Ltd. 保留所有权利。
Numerous studies have demonstrated the ability to identify the body fluid origin of forensic biological stains using messenger RNA (mRNA) profiling. However, the size of the amplimer used in these assays may not be ideal for use with degraded or compromised samples frequently encountered in forensic casework. Therefore, a novel approach to body fluid identification through the use ofmicroRNA (miRNA) profiling was developed. MicroRNAs are only 20-25 bases in length and may be more suitable for use with degraded or environmentally compromised biological samples. We have identified a set of nine differentially expressed miRNAs that permit the identification of the body fluid origin of forensic biological stains using as little as 50 pg of total RNA. (C) 2009 Elsevier Ireland Ltd. All rights reserved.