Roles of PLC-β2 and -β3 and PI3Kγ in chemoattractant-mediated signal transduction
Roles of PLC-β2 and -β3 and PI3Kγ in chemoattractant-mediated signal transduction
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DOI：
10.1126/science.287.5455.1046
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发表时间：
2000-02-11
期刊：
影响因子：
56.9
通讯作者：
Wu, DQ
中科院分区：
文献类型：
--
作者：
Li, Z;Jiang, HP;Wu, DQ
研究了磷脂酰肌醇3-激酶(PI3K)和磷脂酶C(PLC)在缺乏这些关键酶的小鼠化学诱导剂诱导的反应中的作用。PI3Kγ是化学诱导剂诱导产生磷脂酰肌醇3，4，5-三磷酸[Ptdlns(3，4，5)P-3]所必需的，在化学诱导剂诱导的超氧化物的产生和小鼠中性粒细胞的趋化作用以及产生由免疫球蛋白lambda轻链(TL-Ig lambda，L)组成的T细胞非依赖性抗原特异性抗体中起着重要作用。对缺乏PLC-β2和-β3的小鼠的研究表明，PLC通路在趋化物质介导的超氧化物的产生和蛋白激酶的调节中起着重要作用，但对趋化作用没有影响。PLC通路似乎也抑制了某些趋化物质诱导的趋化活性，并抑制了TI-Ig lambda(L)的产生。
The roles of phosphoinositide 3-kinase (PI3K) and phospholipase C (PLC) in chemoattractant-elicited responses were studied in mice lacking these key enzymes. PI3K gamma was required for chemoattractant-induced production of phosphatidylinositol 3,4,5-trisphosphate [Ptdlns (3,4,5)P-3] and has an important role in chemoattractant-induced superoxide production and chemotaxis in mouse neutrophils and in production of T cell-independent antigen-specific antibodies composed of the immunoglobulin lambda Light chain (Tl-Ig lambda(L). The study of the mice Lacking PLC-beta 2 and -beta 3 revealed that the PLC pathways have an important role in chemoattractant-mediated production of superoxide and regulation of protein kinases, but not chemotaxis. The PLC pathways also appear to inhibit the chemotactic activity induced by certain chemoattractants and to suppress TI-Ig lambda(L) production.