Activation of MAP kinase cascade induced by human pancreatic phospholipase A(2) in a human pancreatic cancer cell line
Activation of MAP kinase cascade induced by human pancreatic phospholipase A(2) in a human pancreatic cancer cell line
复制标题
DOI：
10.1016/s0014-5793(97)00373-6
复制
发表时间：
1997-05-05
期刊：
影响因子：
3.5
通讯作者：
Sugiyama, M
中科院分区：
文献类型：
--
作者：
Kinoshita, E;Handa, N;Sugiyama, M
我们发现人胰腺磷脂酶A（2）I组（hPLA（2）-I）诱导的人胰腺癌细胞MIAPaCa-2的生长是通过其特异性受体介导的，而不是通过其催化性质。本研究表明，hPLA（2）-I可诱导MIAPaCa-2细胞中丝裂原活化蛋白激酶（MAPK）级联反应的激活：该消化酶可诱导MEK 1/2、p44/42 MAPK和ATF-2的磷酸化，而MEK选择性抑制剂PD 98059预孵育细胞后，MAPK级联反应中的磷酸化被抑制。此外，该抑制剂剂量依赖性地阻断hPLA（2）-I诱导的MIAPaCa-2增殖，表明MAPK级联的激活对于hPLA（2）-I诱导的MIAPaCa-2增殖是必需的。(C)1997年欧洲生物化学学会联合会。
We have found that the growth of human pancreatic cancer cells MIAPaCa-2, induced by human pancreatic phospholipase A(2) group I (hPLA(2)-I), is mediated via its specific receptor but not via its catalytic property. The present study showed that the activation of mitogen-activated protein kinase (MAPK) cascade in MIAPaCa-2 cells is induced by hPLA(2)-I: this digestive enzyme induced phosphorylation of MEK1/2, p44/42 MAPK and ATF-2, and the phosphorylation in the MAPK cascade was inhibited after the cells were pre-incubated with a selective inhibitor of MEK, PD98059. In addition, this inhibitor dose-dependently blocked the hPLA(2)-I-induced MIAPaCa-2 proliferation, suggesting that activation of the MAPK cascade is essential for the hPLA(2)-I-induced MIAPaCa-2 proliferation. (C) 1997 Federation of European Biochemical Societies.