Radiosensitivity of the cells of an established human melanoma cell line and the parent melanoma xenograft.
Radiosensitivity of the cells of an established human melanoma cell line and the parent melanoma xenograft.
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已建立的人类黑色素瘤细胞系和亲本黑色素瘤异种移植物的放射敏感性。
DOI：
10.1080/09553008314551431
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
T. Brustad
中科院分区：
文献类型：
--
作者：
Einar K. Rofstad;T. Brustad
来自人黑素瘤异种移植物（E.F.）和从该异种移植物建立的细胞系（FME）。在照射前，从指数生长培养物、平台期培养物或无胸腺小鼠（FME-X）的实体瘤中收获已建立细胞系的细胞。在照射期间，细胞保持悬浮在培养基中。在软琼脂中测定细胞的殖民地形成能力。无论是体外还是体内生长，亲代异种移植物的Do值均显著高于已建立的细胞系（p <0.0001）。此外，异种移植物的Dq值显著低于已建立细胞系的指数生长培养物（p <0.05）。因此，建立的细胞系的细胞的辐射反应不代表来自亲本异种移植物的细胞的辐射反应。它的结论是，应非常谨慎地使用已建立的细胞系的存活曲线，试图预测相应的组织学类型的人类肿瘤的放射性。
Survival curves of cells from a human melanoma xenograft (E.F.) and a cell line (FME) established from this xenograft were determined. The cells of the established line were harvested from exponentially growing cultures, plateau phase cultures or solid tumours in athymic mice (FME-X) before irradiation. During irradiation the cells were kept suspended in culture medium. The colony forming ability of the cells was assayed in soft agar. The Do-value was significantly higher for the parent xenograft than for the established line, whether grown in vitro or in vivo (p less than 0.0001). In addition, the Dq-value was significantly lower for the xenograft than for exponentially growing cultures of the established line (p less than 0.05). Thus the radiation response of the cells of the established line was not representative for that of the cells from the parent xenograft. It is concluded that survival curves for established cell lines should be used with great caution in attempts to predict the radiocurability of human tumours of corresponding histological type.
两种异质性人类癌症亚群在体外对 X 射线照射的差异反应。
影响因子：
11.2
作者：
Leith,JT;Dexter,DL;DeWyngaert,JK;Zeman,EM;Chu,MY;Calabresi,P;Glicksman,AS
通讯作者：
Glicksman,AS