Grafting of quantum dots on covalent organic frameworks via a reverse microemulsion for highly selective and sensitive protein optosensing
Grafting of quantum dots on covalent organic frameworks via a reverse microemulsion for highly selective and sensitive protein optosensing
复制标题
DOI：
10.1016/j.snb.2018.04.172
复制
发表时间：
2018-09
期刊：
影响因子：
--
通讯作者：
Tianhong Ni;Dianwei Zhang;Jing Wang;Shuo Wang;Huilin Liu;Baoguo Sun
中科院分区：
文献类型：
--
作者：
Tianhong Ni;Dianwei Zhang;Jing Wang;Shuo Wang;Huilin Liu;Baoguo Sun
采用反相微乳液法结合表面印迹技术合成了CdSe/ZnS量子点（QDs）接枝的共价有机骨架（COFs），并将其用于蛋白质的光传感。COF的独特之处在于，与无机多孔材料相比，它们由具有可调骨架的较轻元素制成。功能性COFs具有广泛的应用前景。本研究利用1，3，5-三甲酰间苯三酚-对苯二胺（TpPa）和3-氨丙基三乙氧基硅烷（APTEs）修饰的CdSe/ZnS量子点通过Schiff碱反应，合成了一种新型的热稳定性和化学稳定性均较好的三维量子点阵列。以TpPa为载体，APTES修饰的量子点为表面活性剂，选择性地、灵敏地检测蛋白质与蛋白质的相互作用，并进一步检测可检测的荧光信号。量子点修饰的COFs具有较高的热稳定性、重复性、选择性和灵敏度，检测限为5.4 × 10− 4 mg/mL。该策略为量子点接枝的COFs的制备提供了一种很有前途的方法，并为量子点接枝的COFs作为蛋白质检测的传感平台提供了巨大的潜力。
A novel reverse microemulsion strategy coupled with surface imprinting technology is developed for the synthesis of CdSe/ZnS quantum dots (QDs)-grafted covalent organic frameworks (COFs) that were used for optosensing protein. COFs are unique in that they are made from lighter elements have tunable skeletons, compared with inorganic porous materials. The functional COFs have become the attractive promising candidates for diversified applications. In this study, a novel thermally and chemically stable three-dimensional crystalline array of QDs grafted on COFs was synthesized, which was performed by Schiff base reactions of 1,3,5-triformylphloroglucinol-P-phenylenediamine (TpPa) and 3-aminopropyltriethoxysilane (APTEs)-modified CdSe/ZnS quantum dots. TpPa was treated as supports to enhance the sensitivity, and APTES modified QDs were employed as tentacle to selectively and sensitively sense the protein-bonding interactions, and further transduce it to the detectable fluorescence signals. The QD-grafted COFs exhibited high thermal stability, repeatability, selective and sensitive optosensing of protein, and the low detection limits was 5.4 × 10−4mg/mL. The proposed strategy provides a promising method for the fabrication of QDs-grafted COFs and the great potential of QDs-grafted COFs as a sensing platform for protein detection.